1 July 2005 – Case of the Week #12

 

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This week’s case is sponsored by InvitrogenThe Zymed® EGFr antibody clone 31G7 is the most widely referenced clone and the industry standard for EGFr detection in formalin-fixed, paraffin embedded (FFPE) tissue samples.  Zymed’s 31G7 antibody is extremely specific for EGFr and does not react with the highly homologous c-erbB-2 (HER2) proteinInvitrogen now offers the Zymed® 31G7 antibody clone as part of a convenient, standardized immunohistochemical kit for the detection of EGFr protein expression in normal and neoplastic tissue.  Invitrogen’s Zymed® EGFr Kit is compatible with manual or automated immunostainers and offers the greatest flexibility with FFPE tissue samples.  For more information, visit www.invitrogen.com/antibodies.  Note: sponsors do NOT have access in any manner to email addresses or other personal information in the possession of PathologyOutlines.com.

 

We thank Dr. Inès Raoelfils, Centre Jean Perrin, Clermont-Ferrand, France for contributing this case.  We invite you to contribute a Case of the Week by emailing NPernick@PathologyOutlines.com with microscopic images (any size, we will shrink if necessary) in JPG or GIF format, a short clinical history and any other images (gross, immunostains, EM, etc.) that you have and that may be helpful or interesting.  We will write the discussion (unless you want to contribute to it).  We particularly need cases of GI, GU and Gynecologic pathology.  We will list you as the contributor, and send you a check for $35 (US) for your time if we use the case.  Please only send cases with a definitive diagnosis. 

 

Case of the Week #12

 

A twenty two year old woman had a large right ovarian mass, which was excised.

 

Micro images: image #1; #2; #3; #4

 

Immunohistochemistry images:  PLAP, AFP, c-Kit / CD117

 

The immunohistochemistry was interpreted as PLAP positive, AFP negative, c-Kit positive.

 

What is your diagnosis? 

 

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Diagnosis:  Dysgerminoma of the ovary

 

Discussion

Dysgerminoma of the ovary is an uncommon tumor, representing less than 1% of ovarian malignancies.  They usually affect young patients, with 80% under age 30 years.  15% have bilateral tumors.  Approximately 5% are associated with gonadal dysgenesis, androgen insensitivity or pseudohermaphroditism.  Some cases appear to represent progression from gonadoblastomas (Virchows Arch 2005 Jun 21 [Epub ahead of print]).  Patients with dysgerminomas typically present with low stage and have a 95% survival.  Metastases can occur to the opposite ovary, retroperitoneal lymph nodes or the peritoneal cavity, but often don’t affect survival.  Dysgerminomas are typically treated with surgery and chemotherapy, and not radiotherapy, which may impair fertility in these usually young patients (National Cancer Institute Treatment Summary).  Dysgerminomas may rarely transform to a yolk sac tumor (Mod Path 1995;8:881), and may be mixed with other germ cell tumors, such as choriocarcinoma, yolk sac tumor or embryonal carcinoma, which worsens the prognosis.  Thus, it is important to adequately section these tumors, and search for different looking areas of the tumor.

 

Dysgerminomas are grossly solid and nodular tumors of variable size.  They are gray-pink, resembling cerebral cortex, and commonly have hemorrhage and necrosis (image).  Microscopically, they are composed of tumor cells separated by fibrous stroma with T lymphocytes.  The tumor cells are large with well defined cell borders.  The cytoplasm contains glycogen, and the nuclei are central, vesicular and contain one or more prominent nucleoli.  Granulomas may be present. 

 

The differential diagnosis includes yolk sac tumor, undifferentiated carcinoma, clear cell adenocarcinoma (usually in post-menopausal women, PLAP negative), lymphoma, melanoma, granulosa cell tumor and metastatic breast carcinoma.  Careful review of the histology of multiple sections, special stains and clinical history are helpful in distinguishing these other tumors.  Dysgerminomas are immunoreactive for PLAP, c-Kit and vimentin.  They may be focally positive for hCG in giant cells, and focally positive for cytokeratin.  They are negative for AFP.  Recent reports indicate that Oct4, a transcription factor involved in the regulation of pluripotency during embryonic development, is sensitive and relatively specific for dysgerminomas (AJSP 2004;28:1341).

 

 

Nat Pernick, M.D.
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