Feature Page
September 2015
Antibodies / Biomarkers

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See the Antibodies / Biomarkers product questionnaire page, for information about companies that have products and services that can help with your antibody and biomarker needs. To add your business or laboratory to applicable Product Questionnaires, please contact Sara Halladay-Klakulak at 248/646-0325 or PathOutSales@gmail.com.

Biomarkers in Proteomics: Deciphering Protein Signatures
Jaleh Mansouri, M.D.

The study of proteins and protein signatures (proteomics) has revolutionized the field of pathology in regard to the role that biomarkers play in the diagnosis and treatment of disease. One of the best-known examples is the expression of estrogen and progesterone receptors and the HER2-neu protein in breast cancer specimens. However, studying proteins in formalin-fixed, paraffin-embedded tissue and other media has represented a technical challenge in proteomics, as proteins are cross-linked during the fixation process. Due to this impediment, protein expression has often been characterized in qualitative and semi-quantitative terms using such techniques as chromogenic and fluorescent immunohistochemistry and in-situ hybridization. Recently, innovations in established methods have facilitated the more accurate quantitation of proteins and biomarkers in fixed tissue and cytology samples.

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) combines high pressure liquid chromatography (HPLC) to fractionate proteins and mass spectrometry (MS) to characterize the composition of these biomolecules. LC-MS/MS is a particularly useful technique in isolating and quantifying proteins that may be hard to extract from a source by other methods. The accurate quantification and identification of proteins in fixed tumor samples is a valuable tool for deciphering the complex protein signatures that underlie so many pathophysiologic processes and may provide great insight for developing diagnostic and therapeutic strategies.

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Advanced Cell Diagnostics, Inc. (ACD) is a leader in the emerging field of molecular pathology. The company's products and services are based on its proprietary RNAscope® technology, the first multiplex fluorescent and chromogenic in situ hybridization platform capable of detecting and quantifying RNA biomarkers in situ at single-molecule sensitivity. ACD partners with pharmaceutical and biotechnology companies to validate biomarkers and to develop companion diagnostic tests for targeted therapeutic development in cancer and other diseases. ACD also pursues internal programs to develop proprietary diagnostic tests in cancer management. For more information please contact info@acdbio.com or call 1-877-576-3636. Learn more at www.acdbio.com.

Ventana Medical is one of the world's leading developers and manufacturers of medical diagnostic instrument and reagent systems providing leading-edge automation technology for use in slide-based diagnosis of cancer and infectious disease. Our products are found in hospital-based histology laboratories, independent reference laboratories and the drug discovery laboratories of some of the world's largest pharmaceutical and biotechnology companies, government labs and medical research centers. For more information please visit our website at www.ventanamed.com or call us at 800.227.2155.

Past / Future Feature Pages:

April 2015: Laboratory Accreditation / Education / CME
May 2015: Computer Software and Systems
June 2015: Diagnostic Testing / Reagents
July 2015: Digital / Imaging / Photography
August 2015: Books & Journals
September 2015: Antibodies / Biomarkers

October 2015: Laboratories
November 2015: Consumable Lab Products / Clinical Lab Analyzers
December 2015: Grossing Equipment / Workstations

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