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September 2012: Antibodies

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Antibodies: Pointing out Pathways in Pathology
Jaleh Mansouri, M.D.

The importance of antibodies in diagnostic pathology has been significant for some time since the introduction of immunohistochemistry, flow cytometry, and clinical immunoassays. Thus far, approximately 350 cell surface antigens alone have been described with corresponding monoclonal antibodies in the cluster of differentiation/designation (CD) system of nomenclature. In addition, knowledge gained in the genetic pathways of tumorigenesis and other disease processes has further expanded the role of antibodies in molecular profiling and prognostication via such methodologies as multiplex tissue microarray. These advances have also led to new ethical issues involving patient care and consent as antibodies can, in some cases, directly point to the likely presence of an underlying genetic defect (e.g. germline defects in DNA mismatch repair enzymes, MSH2, MSH6).

Molecular properties that are critical in determining antibody efficacy include sensitivity and specificity, avidity, level of variability from lot to lot, and structural stability. Other considerations include production costs and clinical applicability. One emerging and exciting area of antibody research involves the development of single-domain antibodies (Nanobody; Ablynx). These (small) molecules are actually monomeric antibody fragments composed of the variable domain, and have increased cellular permeability. This property may further magnify the role of antibodies in pathology as indicators of altered intracellular signaling in various disease states.

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Cell Marque is a quality supplier of a most valuable and innovative portfolio of antibodies suitable for immunohistochemistry on formalin fixed paraffin embedded tissues. Our main focus is on in vitro diagnostics. In the last few years, we have introduced over 100 antibodies to further serve the clinical market. We are passionate about manufacturing quality products. We bring dedication to our customers that can only come from a company whose focus is primarily on antibodies. To learn more about Cell Marque and the products we sell, contact us at 800.665.7284 or visit us online at www.cellmarque.com.

Covance is a provider of gold standard Signet™ immunopathology antibodies, detection chemistries and ancillaries. Monoclonal and polyclonal antibodies are offered for immunohistochemistry on formalin-fixed paraffin-embedded tissues and for a variety of other assays. These antibodies are available in pre-diluted and concentrated formats, providing ease-of-use and reproducibility in both manual and automated staining. Detection chemistries, chromogens, counterstains and buffers are also available in a range of kit volumes to meet your laboratory needs. Covance's antibody portfolio also includes antibodies for neurodegenerative disease, cell biology and a variety custom immunology services. For more information contact Angela Thomas, Immunopathology Product Manager, at 800.223.0796 or angela.thomas@covance.com. Visit our website at www.covance.com.

Epitomics is the world leader in rabbit monoclonal antibody (RabMAb®) technology. Using our proprietary RabMAb technology, we have developed high quality antibodies (EP Clones™) and IHC kits (EpiPrecision™, EpiVision™) for anatomical pathology use. With the benefits of RabMAb technology, EP Clones offers superior binding affinity and specificity. EP Clones have been specifically developed and optimized for use in pathology laboratories for immunohistochemical staining of formalin-fixed paraffin embedded tissues. We are committed to providing high quality products and services that consistently meet the needs of our customers. For more information contact us at 877-772-2622, IVDinfo@epitomics.com, or visit our website at www.epitomics.com/diagnostics.

Leica Microsystems is a leading global developer and manufacturer of a broad range of innovative, high-tech, precision optical systems for the imaging, measurement, and analysis of microstructures. Leica Microsystems is one of the market leaders in each of its four divisions: Life Science, Industry, Biosystems, and Surgical. Business areas include Microscopy and Imaging Systems, Specimen Preparation, Medical Equipment, and Antibodies and Reagents. The company offers instruments and system solutions for the entire histopathology process including the Novocastra™ range of antibodies and reagents, consumable products, and clinical microscopes. The company is represented in over 100 countries with 9 manufacturing facilities in 7 countries, sales and service organizations in 20 countries and an international network of dealers.

Ventana Medical. See what you could be missing. Detect even low-expressing antigens with the Ventana Medical Systems, Inc. (Ventana) Microsatellite Instability biomarkers, powered by the OptiView DAB IHC detection kit on BenchMark IHC/ISH series platforms. Ventana antibodies directed at Mismatch Repair proteins aid in the identification of defects associated with colon carcinoma. Contact your Ventana sales representative to learn more or visit www.ventana.com.

Past / future feature pages:

May 2012: Computer software and systems
June 2012: Diagnostic testing / reagents
July 2012: Imaging / digital / photography
August 2012: Books & Journals

October 2012: Laboratories
November 2012: Consumable Lab Products / Clinical Lab Analyzers
December 2012: Grossing workstations / lab furniture
January 2013: Billing / consulting / management
February 2013: Microscopes / slide imaging
March 2013: Tissue processors / other histology
April 2013: Laboratory Accreditation / Education / CME
May 2013: Computer software and systems
June 2013: Diagnostic testing / reagents
July 2013: Imaging / digital / photography
August 2013: Books & Journals
September 2013: Antibodies

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