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September 2013:
Antibodies / Biomarkers


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Antibodies / Biomarkers: Recombinant Antibodies in Pathology
Jaleh Mansouri, M.D.


The use of antibodies in immunohistochemistry has had a major impact in pathology in the last couple of decades. During this time, the role of immunohistochemistry has evolved from an ancillary technique to a major diagnostic and prognostic tool (e.g. HER2 neu in breast carcinoma). The panel of antibodies available to pathologists has expanded rapidly, due to the development of efficient methodologies for antibody production (e.g. hybridoma). In addition, recombinant antibody technology has become a potentially promising tool in diagnostic pathology.

Recombinant antibodies are produced by a method known as phage display, whereby antibody fragments (e.g. single variable Fv fragments) containing specific binding sites of the parent antibody are cloned and displayed on bacteriophages secreted by infected bacteria (usually Escherichia coli). In this way, antibody “libraries” can be constructed that facilitate isolation of antibody fragments to particular antigens. Advantages of recombinant technology include increased consistency across antibody lots, production of antibodies to highly toxic antigens, and modification of antibody fragments to enhance specific characteristics such as binding affinity. As a result of phage display techniques, “customized” antibodies are becoming increasingly available from biotech companies (e.g. AxioMx, Branford, CT) and will likely complement the trend towards personalized medicine in the coming years.

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Advanced Cell Diagnostics, Inc. (ACD) is a leader in the emerging field of molecular pathology. The company’s products and services are based on its proprietary RNAscope® technology, the first multiplex fluorescent and chromogenic in situ hybridization platform capable of detecting and quantifying RNA biomarkers in situ at single-molecule sensitivity. ACD partners with pharmaceutical and biotechnology companies to validate biomarkers and to develop companion diagnostic tests for targeted therapeutic development in cancer and other diseases. ACD also pursues internal programs to develop proprietary diagnostic tests in cancer management. For more information please contact info@acdbio.com or call 1-877-576-3636. Learn more at www.acdbio.com.

Cell Marque is a quality supplier of a most valuable and innovative portfolio of antibodies suitable for immunohistochemistry on formalin fixed paraffin embedded tissues. Our main focus is on in vitro diagnostics. In the last few years, we have introduced over 100 antibodies to further serve the clinical market. We are passionate about manufacturing quality products. We bring dedication to our customers that can only come from a company whose focus is primarily on antibodies. To learn more about Cell Marque and the products we sell, contact us at 800.665.7284 or visit us online at www.cellmarque.com.

Leica Biosystems is a global leader in workflow solutions and automation, striving to advance cancer diagnostics to improve patients’ lives. Leica Biosystems provides anatomical pathology laboratories and researchers a comprehensive product range for each step in the pathology process, from sample preparation and staining to imaging and reporting. Our easy-to-use and consistently reliable offerings help improve workflow efficiency and diagnostic confidence. The company is represented in over 100 countries. It has manufacturing facilities in 7 countries, sales and service organizations in 19 countries, and an international network of dealers. The company is headquartered in Nussloch, Germany. Visit www.LeicaBiosystems.com for more information.

Ventana Medical is one of the world’s leading developers and manufacturers of medical diagnostic instrument and reagent systems providing leading-edge automation technology for use in slide-based diagnosis of cancer and infectious disease. Our products are found in hospital-based histology laboratories, independent reference laboratories, and the drug discovery laboratories of some of the world’s largest pharmaceutical and biotechnology companies, government labs, and medical research centers. For more information please visit our website at www.ventanamed.com or call us at 800.227.2155.

Past / Future Feature Pages:

May 2013: Computer Software and Systems
June 2013: Diagnostic Testing / Reagents
July 2013: Imaging / Digital / Photography
August 2013: Books & Journals
September 2013: Antibodies / Biomarkers

October 2013: Laboratories
November 2013: Consumable Lab Products / Clinical Lab Analyzers
December 2013: Grossing Equipment / Workstations
January 2014: Billing / Consulting / Management
February 2014: Microscopes / Microscopy Products
March 2014: Tissue Processors / Other Histology
April 2014: Laboratory Accreditation / Education / CME
May 2014: Computer Software and Systems
June 2014: Diagnostic Testing / Reagents
July 2014: Imaging / Digital / Photography
August 2014: Books & Journals
September 2014: Antibodies / Biomarkers

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