Molecular Pathology

Authors/Editors: Zubair W. Baloch, M.D., Ph.D., Joshua Bradish, M.D., Betty Chung, D.O., M.P.H., M.A., Rodney E. Shackelford, D.O., Ph.D.; Editors: Liang Cheng, M.D., Gregory A. Hosler, M.D., Ph.D. (see Authors page)

Revised: 1 September 2015, last major update IN PROGRESS

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Table of contents

DNA purification: introductionanalyzing puritybasic protocolanion exchange chromatographycesium chloride density gradient centrifugationcommercial DNA extraction machinesethanol precipitationorganic extractionPCR inhibitorsRNA purificationsilica adsorptiontissue preparation

DNA sequencing: historyMaxam-Gilbert sequencingSanger sequencingcapillary electrophoresisother innovationsreal timepyroseqencingnanotechnologyRoche 454 FLX pyrosequencerIllumina Genome AnalyzerHeliScope Sequencer

FISH: generalprobesprotocolprobe patternsimages

Microarray: introductionhistorybasicsconsiderationserrors
variations:antibodybead basedcellularCGHsolid phasetissue (TMA)

PCR: definitionhistorybasicsTaq polymerasereaction stagesthermocyclersapplications
variations:generalmethylation specificmultiplexnestedreal-timereverse transcriptase

Molecular Cytology competency assessment: overviewbasic principlesgene organizationinherited diseasesidentity testingbasic molecular oncologytechniquesclinical applicationsystem based monitors

Inherited Disease Molecular Testing: Lynch syndrome

Melanoma mutations: ARID2BAP1BRAFGNAQHRASKITNF1NRASPTEN



Recent Molecular Pathology books
 

Best: 2012

Burtis: 2014

Cheng: 2013

Coppola: 2013

Harbers: 2014

Highsmith: 2013

Idowu: 2015

Kulkarni: 2014

Netto: 2014

Pecorino: 2012

Sepulveda: 2013

Tan: 2013

Wright: 2013

Yousef: 2014



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