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CD88: C5A and its effects
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CD82: endometrial carcinoma
CD82: oral cavity (normal and malignant)
CD82: breast carcinoma (D-F)
CD83: infantile hemangioma endothelium
CD83: decidua
CD83+ dendritic cells in breast tumor
CD87: endometrial adenocarcinoma
CD87: pancreatic adenocarcinoma (figures B, D)
CD88: normal and Alzheimer brain
Contributed by Rola Saleeb, M.D., Ph.D.
Normal ABCC2 in tubules
Negative ABCC2 in PRCC
PRCC cytoplasmic
Cytoplasmic ABCC2 in PRCC
PRCC low
Low brush border pattern ABCC2
Papillary RCC, prominent atypia
High brush border ABCC2 expression
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Cryptosporidium:
Oocysts: modified acid-fast stain
Stool specimen (Ziehl-Neelsen)
Oocysts: auramine-rhodamine stain
Isospora:
Acid-fast stain
Mycobacterium leprae:
Liver (Fite stain)
Mycobacterium tuberculosis:
Ziehl-Neelsen stains
Auramine stain of lung
Skin biopsies
Mycobacterium avium complex:
Site-unknown, breast and colon (Ziehl-Neelsen)
Nocardia:
Fite-Faraco modified acid fast stain of lung
Other:
Tuberculous lymphadenopathy (Ziehl-Neelsen)
Pleural fluid
Acid fast stain
Contributed by Chunyu "Hunter" Cai, M.D., Ph.D.
Adult onset acid maltase deficiency
Central core myopathy
Cystinosis distal myopathy
Degenerating myofibers
Hydroxychloroquine myopathy
Inclusion body myositis
Infant onset acid maltase deficiency
Lupus myositis
Myophagocytosis
Normal muscle
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Helical structure of F-actin
G-actin to F-actin transition
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Various images
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Endometriosis
Myofibroblastoma: breast (fig d)
Myofibroblastoma: lymph node
Rhabdomyo-sarcoma: CNS, embryonal (fig 4)
Rhabdomyo-sarcoma: oral cavity
Contributed by Kemal Kösemehmetoğlu, M.D.
Leiomyoma
SMA expression in leiomyoma
Leiomyosarcoma
Diffuse cytoplasmic block SMA expression
Nodular fasciitis
SMA in nodular fasciitis
Myxofibrosarcoma
Focal SMA expression in myxofibrosarcoma
Glomus tumor
SMA in glomus tumor
Undifferentiated pleomorphic sarcoma
Focal SMA in undifferentiated pleomorphic sarcoma
Inflammatory myofibroblastic tumor
Myofibroblastic type SMA in IMT
Atypical apocrine adenosis
SMA in myoepithelial layer of breast adenosis
Radial scar / complex sclerosing lesion
SMA in radial scar / complex sclerosing lesion
Sclerosing papilloma
Pseudoinvasion in sclerosing papilloma
SMA positive myoepithelial layer
Tram track (myofibroblastic) staining pattern
Block (smooth muscle-like) staining pattern
Contributed by Vikas Mehta, M.D., Maria M. Picken, M.D., Ph.D. and Cullen Lilley, M.S., M.A.
Small bowel adenovirus infection
Coagulative hepatocyte necrosis
Lung biopsy
Renal parenchyma
Contributed by Maria M. Picken, M.D., Ph.D.
Crystalline array
Images hosted on other servers:
Left: normal liver; right: negative control
Liver and hepatocellular carcinoma
AFIP images and Cases #78, 94 and 110
Bladder: urothelial
carcinoma with
gland-like lumina
Esophagus
Mucin stains of Barrett mucosa
Mucin stains of Barrett mucosa
Heart: myxoma with glandular structures
Kidney: adenocarcinoma (AB-PAS)
Sacrum, Chordoma: stroma stains with Alcian blue
Scrotum: Paget disease
Thyroid gland: signet ring cell variant
Images hosted other servers:
Breast:
mucoepidermoid
carcinoma
(Alcian blue-PAS)
Esophagus, Barrett: GE junction
Eye: macular corneal dystrophy
Gallbladder: pyloric metaplasia
Kidney: mucinous
tubular and
spindle cell
carcinoma
Soft tissue: proliferative fasciitis - AB-PAS
Images hosted on other servers:
ALK signalling pathway
Contributed by A. Cristina Vargas, M.B.B.S., Ph.D., Patricia Guzman, M.D., Fiona Bonar, M.B.B.Ch., Alison Cheah, M.B.B.S. and Martin Jones, M.B.B.S.
ALK rearranged lung adenocarcinoma
ALK IHC in lung adenocarcinoma
Anaplastic large cell lymphoma
ALK IHC in ALCL
Anaplastic large cell lymphoma
ALK IHC in ALCL
Uterine IMT
ALK IHC on uterine IMT
ALK-EML4 spindle cell tumor
ALK-EML4 spindle cell tumor ALK
Undifferentiated pleomorphic sarcoma with ALK
Undifferentiated pleomorphic sarcoma-like ALK
Epithelioid fibrous histiocytoma
ALK IHC in epithelioid fibrous histiocytoma
FUS-TFCP2
rearranged
rhabdomyosarcoma
ALK IHC on
FUS-TFCP2
rhabdomyosarcoma
Contributed by A. Cristina Vargas, M.B.B.S., Ph.D.
ALK break apart FISH
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Break apart signal patterns for ALK rearrangement
Contributed by Chunyu "Hunter" Cai, M.D., Ph.D.
Normal muscle
Dermatomyositis Mi2
Dermatomyositis NXP2
Antisynthetase syndrome PL7
Antisynthetase syndrome Jo1
Necrotizing autoimmune myopathy HMGCR
Reducing body myopathy
Contributed by João Lobo, M.D., Rui Henrique, M.D., Ph.D.
Prepubertal testicular yolk sac tumor
Prepubertal testicular yolk sac tumor, AFP
Prepubertal testicular yolk sac tumor
Prepubertal testicular yolk sac tumor, AFP
Metastatic yolk sac tumor
Metastatic yolk sac tumor, AFP
Testicular mixed germ cell tumor
Testicular mixed germ cell tumor, AFP
Fetal liver
Fetal liver, AFP
Hepatoblastoma
Hepatoblastoma, AFP
Contributed by Avani Pendse, M.D., Ph.D.
Eosinophilic intracytoplasmic globules
PASD positive intracytoplasmic globules
Alpha-1 antitrypsin IHC
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Breast: primary acinic cell carcinoma (fig F)
Skin: nevi and melanoma
Stomach: hepatoid
adenocarcinoma
(fig C)
Contributed by Meenakshi Vij Gupta, M.D.
Lewy body in Parkinson disease
Cellular inclusions and neuritis plaques
Contributed by Jonathan Epstein, M.D., Debra Zynger, M.D.
Prostate adenocarcinoma, triple stain
RCC papillary type 1 with AMACR
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Enhancement of
formic acid mediated
amyloid beta peptide
antigen retrieval
IHC of beta
amyloiddeposits
in the cerebral cortex
and blood vessels
Contributed by Monika Roychowdhury, M.D.
Skin metastatic breast carcinoma 20x
Androgen receptor
in metastatic breast
carcinoma 20x
Skin metastatic breast carcinoma 40x
Androgen receptor
in metastatic breast
carcinoma 40x
Case #214
Salivary gland AR-
low grade cribriform
cystadenocarcinoma
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Breast: apocrine DCIS
Breast: apocrine metaplasia
Prostate carcinoma
metastatic to bone
Contributed by GenomeMe and Cell Marque Corporation
Colon (normal), clone IHC400
Liver (normal), clone IHC400
HCC - nuclear and cytoplasmic staining
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Hepatocellular carcinoma
Moderately differentiated HCC
Liver: benign and hepatocellular carcinoma
Metastatic colonic adenocarcinoma to liver
Cholangiocarcioma
Lung: TB related granulomas
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Gene map, regulation of chromatin structure
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Cervix: borderline seromucinous tumors
Ovary: clear cell carcinoma (A, B)
Ovary: endometriotic cyst and associated well-differentiated endometrioid carcinoma
Stomach: gastric carcinoma
Uterus: endometrioid carcinoma
Uterus: endometriosis (G-I)
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Neuroblastoma
Pancreas: ALT+ tumors
Pancreas: MEN1
microadenoma and
pancreatic neuroendocrine
tumor
Images hosted on other servers:
ATM in DNA
damage response
ATM signaling network
Contributed by Joo-Shik Shin, M.B.B.S., Ph.D.
Rectal adenocarcinoma
Images hosted on other servers:
Bronchioalveolar carcinoma
of lung: A-H&E, B-cytoplasmic
staining for CEA, C-cytoplasmic
staining for B72.3
Images hosted on other servers:
BAP1 protein localization
BAP1 interactions
Contributed by Gustav Stålhammar, M.D., Ph.D.
Uveal melanoma, BAP1 positive
Uveal melanoma, BAP1 negative
Uveal melanoma, BAP1 mixed
AFIP images
BCL2 translocation in follicular lymphoma
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2 main pathways to apoptosis
Contributed by Vladimir Osipov, M.D. (Case #171), Keith Kaplan, M.D. (Case #150) and AFIP
Secondary follicle
Follicular lymphoma
Burkitt lymphoma
Spindle cell epithelioma of vagina
Synovial sarcoma: right pericardium
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Various images
Collecting duct carcinoma-kidney
Follicular lymphoma
Follicular lymphoma of thyroid: t(14;18) negative / BCL2 negative (left) versus positive (right)
Solitary fibrous tumor
Contributed by Elena M. Fenu, M.D.
Staining algorithm for DLBCL
Contributed by Elena M. Fenu, M.D.
Reactive follicular hyperplasia
Follicular lymphoma, grade 1 - 2
Follicular lymphoma, grade 3B
Angioimmunoblastic T cell lymphoma
Burkitt lymphoma
T cell / histiocyte rich large B cell lymphoma
Nodular lymphocyte predominant Hodgkin lymphoma
Classic Hodgkin lymphoma
Contributed by Isidro Machado, M.D., Ph.D.
Undifferentiated round cell sarcoma
Undifferentiated round cell sarcoma, BCOR positive
Contributed by Jijgee Munkhdelger, M.D., Ph.D. and Andrey Bychkov, M.D., Ph.D.
Ovarian serous carcinoma immunoprofile
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Basaloid squamous cell carcinoma is BerEP4+
Breast: ductal and
lobular carcinoma -
primary and
metastases
(EpCAM)
Breast cancer: increased EpCAM expression in metastases
Colon: loss of EPCAM expression in cancers associated with Lynch syndrome caused by heterozygous EPCAM germline deletions
Esophagus: normal
and Barrett metaplasia;
gastric mucosa
Unknown site: adenocarcinoma (MOC31)
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Reactive mesothelial cells (BerEP4 / MOC31 negative) versus metastatic adenocarcinoma (positive)
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Cadherins and catenins in signal transduction
Role of β catenin in cell
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Colon: serrated adenoma
Colon: normal and carcinoma
Liver: metastatic
colorectal carcinoma
in sentinel lymph
node and liver
Soft tissue: liposarcoma
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Cadherins and catenins in signal transduction
Role of beta catenin in cell
Contributed by Pamela Wirth, Ph.D. (source: University of Toronto and Human Protein Atlas)
Normal pancreatic tissue
Cervix
Breast carcinoma
Colorectal carcinoma
Lung cancer
Normal kidney
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Normal tonsil
Various B cell lymphomas
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MAPK signaling pathway
Contributed by Andrew Colebatch, M.B.B.S., Ph.D. and Andrey Bychkov, M.D., Ph.D.
BRAF V600E positive nevus
BRAF V600E positive melanoma
V600E mutant protein is diffusely expressed in tumor / cancer but not normal tissue
Diffuse cytoplasmic staining
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MAPK and AKT pathways
Resistance diagram
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Medullary thyroid cancer
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Normal mesothelium
Various images and quality control
Mucinous cystic neoplasm of mesentery
Contributed by Andrey Bychkov, M.D., Ph.D.
Cluster of C cells
Pericapillary location of C cells
Heavy background
in the immediate
stroma and follicles
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Thyroid
Single endocrine cell
Prostate
(figure 1)
HGPIN (figure 2)
Bone metastasis (figure 3)
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Malakoplakia of bladder (von Kossa calcium stain)
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Colon-pericrytal fibroblast sheath (fig A)
Leiomyosarcoma, soft tissue
Case #62
Sclerosing lobular hyperplasia
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Breast:
adenomyoepithelioma
MFH of bone
(fig B, F)
Contributed by Nick Baniak, M.D. and Sharon Bihlmeyer, M.D.
Sarcomatoid mesothelioma
Sarcomatoid mesothelioma (CK5)
Sarcomatoid mesothelioma (calretinin)
Ganglion cells in colon
No ganglion cells
Ovarian granulosa cell tumor (adult)
Contributed by Nick Baniak, M.D.
Lung adenocarcinoma
Mesothelioma
Contributed by Nick Baniak, M.D.
Clear cell RCC
Clear cell papillary RCC
Papillary RCC
Negative renal parenchyma
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Classification examples
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Bone: osteoclasts in osteoclastoma and normal
Intervertebral disc
Maxillary sinus: basaloid squamous cell carcinoma
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CCR2: lamina propria lymphocytes
CCR5: HIV entry into CD4+ cell via CCR5 co-receptor
CCR6: immature
dendritic cells
traffic to lung via
CCR6 / CCL20 axis
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CCR2: human glomeruli
CCR3:
Atherosclerosis
Eosinophils and airway epithelium
Lymph node: Hodgkin disease
Skin: cutaneous T cell lymphoma
Paraffin embedded malignant melanoma
Renal cell carcinoma
CCR4:
Lymph node: normal
Lymph node and peripheral blood: adult T cell leukemia / lymphoma
Skin: Sézary Syndrome
CCR6:
Lung: nonsmall cell lung carcinoma
Lung: sarcoidosis
Lymph node: melanoma
Spleen: memory T cells
Tonsils: inflamed
CCR7:
Breast: various carcinomas
Colorectum: T cells in advanced adenocarcinoma
Esophagus: squamous cell carcinoma and metastatic lymph node
Lymphoma T cell
Skin: melanoma and sentinel lymph node
CCR9:
Ovary: normal and carcinoma
Images hosted on other servers:
CD182: ovarian carcinoma
CD185: chronic H. pylori gastris (left); MALT lymphoma (right)
CD185: AIDS related non-Hodgkin lymphoma
CD185: synovium of nonrheumatoid arthritis (left) and rheumatoid arthritis (right)
CCR2: human glomeruli
CCR3: eosinophils and airway epithelium
CCR3: lymph nodes involved by Hodgkin disease
CCR3: cutaneous T cell lymphoma
CCR3: paraffin embedded malignant melanoma
CCR3: renal cell carcinoma
CCR4: lymph node normal
CCR4: lymph node (adult T cell leukemia / lymphoma)
CCR4: Sézary syndrome
CCR6: nonsmall cell lung carcinoma
CCR6: lung sarcoidosis
CCR6: lymph node melanoma
CCR7: various breast carcinomas
CCR7: T cells in advanced adenocarcinoma in colorectum
CCR7: esophageal SCC and metastatic lymph node
CCR7: lymphoma T cells
CCR7: skin melanoma and sentinel lymph node
CCR9: normal and carcinoma ovaries
CD247: normal expression
CD247: reduced expression
CD248: IHC detection of FB5 antigen
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CCR4:
peripheral blood
(adult T cell
leukemia / lymphoma)
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CD1d: antigen presentation
CD1d: viral evasion of antigen presentation
CD1d: intestinal epithelial CD1d expression
CD2: T cells and antigen presenting cells
CD9: structure
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CD1: primary and metastatic melanoma
CD1: Langerhans cells of anal mucosa
CD1b: leprosy patient
CD1c: marginal zone B cells (spleen)
CD1c: stomach: H. pylori+ mucosa
CD1c: thyroid gland
CD1c: immature dendritic cells
CD1d: HPV associated lesions (cervix)
CD1d: trophoblast (placenta)
CD1d: scalp skin
CD2: hydroa
vacciniforme-like
lymphoma
Brain (CD6-)
CD6: colon
Kidney (CD6-)
Skeletal muscle (CD6-)
CD6: tonsil
Uterus (CD6-)
CD7: normal tonsil
CD7: cutaneous T cell lymphoma
CD9: normal tissue (cerebrum)
CD9: normal tissue (cervical squamous epithelium)
CD9: cervical carcinoma
CD9: CNS nonneuroepithelial tumors
CD9: well differentiated adenocarcinoma (gallbladder)
CD9: mesothelioma
CD9: basal and
squamous cell
carcinoma and
actinic keratosis
CD9: small cell lung cancer
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CD1c: various cellular locations
Contributed by Bradford Siegele, M.D., J.D.
Lymphoblasts in B ALL / LBL
Lymphoblasts in B ALL / LBL, PAX5+
Lymphoblasts in B ALL / LBL, CD10+
Lymphoblasts in B ALL / LBL, TdT+
Starry sky of Burkitt lymphoma
Burkitt lymphoma cells, CD20+
Burkitt lymphoma cells, CD10+
Burkitt lymphoma cells, BCL6+
Burkitt lymphoma cells, TdT-
Large atypical cells of DLBCL
Large atypical cells of DLBCL, OCT2+
Large atypical cells of DLBCL, CD10+
Large atypical cells of DLBCL, BCL6+
Large atypical cells of DLBCL, MUM1 variable
Atypical cells and blood vessels of AITL
Atypical cells of AITL, CD3+
Atypical cells of AITL, CD4+
Atypical cells of AITL, CD8-
Atypical cells of AITL, CD10+
Atypical cells of AITL, ICOS+
Nodules of atypical cells of FL
Nodules of atypical cells of FL, CD20+
Nodules of atypical cells of FL, CD10+
Nodules of atypical cells of FL, BCL2+
Nodules of atypical cells of FL, BCL6+
Atypical follicular
dendritic cell
meshworks of
FL, CD21+
Pseudopapillae of SPN
Pseudopapillae of SPN, CD10+
Pseudopapillae of SPN, alpha-1 antitrypsin+
Endometriosis of sigmoid colon
Endometriosis of sigmoid colon, CD10+
Endometriosis of sigmoid colon, ER+
Moderately atypical spindle cells of ESS
Spindle cells of ESS, CD10+
Minimally atypical cells of ccRCC
Atypical cells of ccRCC, CD10+
Atypical cells of ccRCC, CAIX+
Atypical cells of ccRCC, vimentin+
Atypical cells of ccRCC, CK7-
Atypical cells of ccRCC, CD117-
Atypical cells of ccRCC, CD15-
Contributed by Jijgee Munkhdelger, M.D., Ph.D. and Andrey Bychkov, M.D., Ph.D.
Myoepithelial layer on CD10
Breast tubular adenoma immunoprofile
Contributed by Bradford Siegele, M.D., J.D. and Aashish Khatri, M.L.S., M.B.A.
Flow cytometric analysis, B ALL / LBL
Flow cytometric analysis, T ALL / LBL
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Flow cytometric analysis, DLBCL
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CD14: toll-like receptor pathways
Membrane bound and serum forms of CD14
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CD11a: intestinal mucosa (fig F)
CD11a: eruptive papules with efalizumab therapy
CD11b: normal spleen
CD11b: gastric cancer
CD14: normal bone marrow (fig C)
CD14: acute monoblastic leukemia (fig F) (bone marrow)
CD14: acute myelomonocytic leukemia (fig F) (bone marrow)
CD14: microglia in Alzheimer patients (CNS)
CD14: biliary atresia (liver)
Anti CD15s
antibody blocks
infection of HL60
cells by HGE agent
CD15s expression correlates with HL60 cells
CD15u: colorectal cancer
CD15u: skin
CD18: fibrocytes
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CD100: head and neck squamous cell carcinoma
CD107b: loss of staining in muscle and heart
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Domain structure of the αE(CD103)β7 integrin
Contributed by Maurice Richardson, M.D. and Moiz Vora, M.D.
Diffuse lymphoid infiltrate
CD103+ neoplastic lymphocytes
Intrasinusoidal lymphoid infiltrate
CD103+ neoplastic lymphocytes
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Hepatocellular carcinoma and normal liver
Lung - non small cell carcinoma (fig D)
Contributed by Debra L. Zynger, M.D. and Andrey Bychkov, M.D., Ph.D.
Seminoma with IHC
Testis
GIST
Contributed by Joseph Khoury, M.D.
Tonsil
Blastic plasmacytoid dendritic cell neoplasm
Contributed by Leica Microsystems
Prostate: normal
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CNS: glioblastoma & colon: carcinoma
Colon: normal
Liver: left-normal, right-hepatocellular carcinoma
Prostate: carcinoma
Skin (inflammatory), tonsil
Various tumors
Contributed by Pamela Wirth, Ph.D. (source: University of Toronto and Human Protein Atlas) and Yuri Tachibana, M.D.
Bone marrow biopsy
Spleen
Breast carcinoma
Colon carcinoma
Liver carcinoma
Prostate carcinoma
Chronic endometritis
Contributed by Raghava Munivenkatappa, M.D. (Case #48) and AFIP
Paranuclear / Golgi staining pattern
Nodular lymphocyte predominant
Renal cell carcinoma: papillary type
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Small intestine: normal Paneth cells (fig B)
Colon: invasive colorectal carcinoma
Contributed by Charles J. Sailey, M.D.,
Borislav A. Alexiev, M.D. and
John C. Papadimitriou, M.D.
CD163: histiocytic sarcoma
Contributed by
Jijgee Munkhdelger, M.D., Ph.D.
and Andrey Bychkov, M.D., Ph.D.
CD163: juvenile xanthogranuloma immunoprofile
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CD182: inflammatory infiltration
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CD182: ovarian carcinoma
CD185: chronic H. pylori gastris (left); MALT lymphoma (right)
CD185: AIDS related non-Hodgkin lymphoma
CD185: synovium of non-rheumatoid arthritis (left) and rheumatoid arthritis (right)
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CD19 structure and signaling
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Langerhans cell histiocytosis:
Brain
Lung: right is bronchioalveolar lavage
Skin
Other:
Esophagus: Barrett metaplasia
Oral cavity: gingiva - normal and diseased
Skin: mycosis fungoide
Case #72
Langerhans cell histiocytosis:
Skull - cytology
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Langerhans cell histiocytosis: Birbeck granules
Contributed by Leonie Frauenfeld, M.D., Andrey Bychkov, M.D., Ph.D. and Kaveh Naemi, D.O.
Germinal center with strong CD20 positive B cells
SLL / CLL infiltrate in a lymph node
Hairy cell leukemia infiltrate in the bone marrow
MALT lymphoma
Burkitt lymphoma
of ileocecal valve
Cases #101, 118, 127, 130, 284 and AFIP images
Lymph node: angiomyomatous hamartoma
Skin: halo nevus
MALT lymphoma of stomach
Myeloid sarcoma of bone (negative)
NK / T cell lymphoma, nasal type (tumor cells are negative)
T cell lymphoma
Hodgkin lymphoma, nodular lymphocyte predominant subtype
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CD28: costimulation
Competition between CTLA4 and CD28 for same ligands
CD28 costimulation is also required for proper memory cell activation
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CD22: high grade B cell lymphoma, fig F
CD22: B-ALL, fig D
CD22: Burkitt lymphoma, fig D
CD22: normal tonsil
CD24: bladder carcinoma
CD24: colon, lung, ovary (left to right)
CD24: DCIS (breast)
CD24: extrahepatic bile duct carcinoma
CD24: salivary gland mucoepidermoid carcinoma
CD25: HTLV1+ adult T cell leukemia / lymphoma
CD25: mastocytosis
CD26: normal colon
CD26: proximal tubules (kidney)
CD26: prostate carcinoma
CD29: lung cancer
AFIP images and Cases #81, 179 and 284
Follicular dendritic cell sarcoma
Littoral cell angioma of spleen
Lymph node
Images hosted on other servers:
Mediastinum
Tonsil
Nodal marginal zone B cell lymphoma
Contributed by Mihaly Sulyok, M.D., Ph.D. and Christian M. Schürch, M.D., Ph.D.
CLL / SLL
Follicular lymphoma
Tonsilla
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CD247: T cell activation
Images hosted on other servers:
CD247: normal expression
CD247: reduced expression
CD248: IHC detection of FB5 antigen
Contributed by Christian Schürch, M.D., Ph.D.
Immunohistochemical
double staining
(CD27 and CD34)
Contributed by Frido Bruehl, M.D. and Betty Gay, B.S.
Flow cytometry of CVID patient
Contributed by Leonie Frauenfeld, M.D.
CD3+ mature T cells in lymphadenitis
CD3+ neoplastic cells in PTCL, TBX21
Case #36
Soft tissue: anaplastic large cell lymphoma
Contributed by Mario L. Marques-Piubelli, M.D. and Roberto N. Miranda, M.D.
Activation of MAPK / ERK1 / ERK2 and NFκB pathways
Contributed by Roberto N. Miranda, M.D.
ALK+ anaplastic large cell lymphoma (ALCL) in lymph node
Primary cutaneous ALCL
Breast implant associated ALCL
Lymphomatoid papulosis type C
Peripheral T cell lymphoma, NOS, in colon
Primary mediastinal large B cell lymphoma
Mediastinal gray zone lymphoma
Nodular sclerosis classic Hodgkin lymphoma, syncytial variant
Kikuchi-Fujimoto disease
Dermatopathic lymphadenopathy
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CD36 and macrophage phagocytosis
Case #81
CD35: follicular dendritic cell tumor
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CD35: liver mass
CD39: donor liver
Cases #107, 187, 77
Retiform hemangioendothelioma
Kidney, glomus tumor
Epithelioid
hemangioendothelioma
Images hosted on other servers:
Normal structure:
Glomeruli (fig A)
Alveoli (fig A)
Spleen (fig A)
Liver (fig A)
Lymph nodes (fig A)
Skin (fig A)
Benign vascular tumors / processes:
Skin - diffuse dermal angiomatosis
Skin - epithelioid hemangioma
Malignant vascular tumors:
Kaposi sarcoma
Paratesticular
Lymphovascular invasion:
Breast
Colorectal carcinoma
Uterus, endometrial carcinoma
stage I
Uterine leiomyosarcoma
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CD4+ T cell and antigen presenting cell
CD4 acting as HIV receptor
Images hosted on other servers:
CNS: acute demyelinating disease (fig 12)
Joint: rheumatoid arthritis (fig B)
Lymph node: atypical paracortical hyperplasia (fig B)
Salivary gland: Sjögren syndrome (fig B)
Small bowel: T cell lymphoma (left: fig D; middle: fig D); right - stomach (fig C)
Soft tissue: inflammatory myopathy
Case #36
Soft tissue mass: anaplastic large cell lymphoma
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CD42a - d complex
CD42b: interactions
CD45RB expression changes during germinal center reaction
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CD40: soft tissue sarcomas
CD45RO: multiple myeloma plasma cell staining
Cases #140 and #127
Leukemia cutis
MALT lymphoma of stomach
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Mantle cell lymphoma
Anaplastic large cell lymphoma
Images hosted on other servers:
CD44 molecule structure with ligands
Contributed by John Yahya I. Elshimali, M.D. and Maria Tretiakova, M.D., Ph.D.
Epithelium of fallopian tube
Fallopian stromal cells
CD44 positive epithelial cells in fallopian tube
CD44 positive epithelial cells in fallopian tube
Positive CD44 expression in fallopian tube epithelium
Decidual and stromal cells in fallopian pregnancy positive for CD44
Normal bladder mucosa
CD44 - full thickness in normal bladder mucosa
Bladder CIS
CD44 - loss of CD44 expression
Pagetoid CIS vs reactive inflammation
CD44 - patchy loss of expression in pagetoid CIS
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CD44 transmembrane receptor function
Contributed by Elena M. Fenu, M.D.
Anaplastic large cell lymphoma
Diffuse large B cell lymphoma
Plasmablastic lymphoma
Leukemia cutis
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Intraepidermal T cells in fixed drug eruption lesions (fig d)
Papulonodular lesion show dermal, intraepithelial neoplastic lymphocytes
Contributed by Frido Bruehl, M.D.
Proposed mechanism of action of CD47
Contributed by Christian M. Schürch, M.D., Ph.D.
Mesothelioma, cell block, CD47
Contributed by Julia Braza, M.D. and Cases #281 and #17
Breast CLL / SLL
Eyelid: mantle cell lymphoma
Thymic carcinoma,
nonkeratinizing
squamous cell
subtype
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Tumors / tissue typically CD5+:
Lymph node: mantle cell lymphoma (fig B)
Lymph node: SLL
Tumors / tissue occasionally / rarely CD5+:
Lymph node: follicular lymphoma: typically CD5 stains only adjacent T cells (left); rarely tumor is CD5+ (right)
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CD50: normal
uterine cervix
and cervical
cancer tissues
CD52: cryostat
sections stained
with biotinylated
Campath-1H
CD52: paraffin
wax sections
stained with
Campath-1G
CD55: breast carcinoma staining
CD55: breast carcinoma staining
CD58: buccal
mucosa with
intense LFA 3+
staining
CD58: cardiac muscle with LFA-3+ staining
CD58: cerebellum, liver, skin
CD58: proximal tubule and distal tubule (kidney)
CD58: cells near
seminiferous tubule
boundary (testis)
CD59: diabetic and nondiabetic:
kidneys (left), nerves (right)
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Large granular lymphocytic leukemia (fig 11)
Papillary carcinoma of thyroid (fig B)
"Atypical cytology, cannot exclude papillary carcinoma"
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CD66: colon
CD66: expression at maternal fetal surface
CD66: various breast lesions
Implanted
ovum (CD66f+,
syncytiotrophoblast)
Normal placenta (CD66f+, trophoblast)
Hydatidiform mole
(syncytiotrophoblast)
Invasive mole
(CD66f+, large
trophoblast cells)
Choriocarcinoma
of uterus (CD66f+,
syncytiotrophoblast)
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Superfamily of integrins
Contributed by Pamela Wirth, Ph.D. (sources: University of Toronto and Human Protein Atlas)
Bone marrow biopsy
Liver
cholangiocarcinoma
Kidney adenocarcinoma
Lung squamous cell carcinoma
Colon carcinoma
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Cellular neurothekeoma
Contributed by Frido Bruehl, M.D.
Fibrolamellar carcinoma of the liver
Kikuchi-Fujimoto disease of the lymph node
Reticulohistiocytoma of the skin
Contributed by Christian Schürch, M.D., Ph.D.
Bone marrow biopsy
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CD74: renal tumors (figs D-F)
CD74: multiple myelomas
(figs G-J)
CD74: increased expression in H. pylori+ epithelium
CD75: marginal zone lymphoma (aberrant expression)
Contributed by Luca Morelli, M.D. and Claudio Luchini, M.D., Ph.D.
CD71 in normal bone marrow
CD71 in bone marrow in myelodysplastic syndrome
CD71 in bone marrow in AML M6
CD71 in placental tissue
Glycophorin staining
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CD8+ T cell activation requires 2 signals
Case #179
Splenic littoral cell angioma, sinus lining cells are CD8-
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Bladder: follicular cystitis (fig D)
Skin: cutaneous CD8+ epidermotropic T cell lymphoma
Skin: lymphomatoid papulosis (fig B, C)
Case #36
Anaplastic large
cell lymphoma:
partial CD4 / CD8
coexpression
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Structure
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Normal tissue:
Cerebrum
Cervical squamous epithelium (fig A)
Various tumors:
Cervical carcinoma
CNS nonneuroepithelial tumors
Gallbladder: well differentiated adenocarcinoma
Mesothelioma
Skin: basal and
squamous cell
carcinoma and
actinic keratosis
Small cell lung cancer
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CD90: normal and diseased skin
CD93: various images
CD93: liver (figure 6B)
CD94: normal tonsil
CD97: normal oral mucosa
CD98: placenta (fig 4)
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Cytotoxic T lymphocyte pathway
Contributed by Frido Bruehl, M.D.
CD99 in Ewing sarcoma
CD99 in T cell acute lymphoblastic leukemia
CD99 in synovial sarcoma
CD99 in CIC-DUX rearranged sarcoma
EWSR1-NFATC2 rearranged sarcoma
EWSR1-NFATC2 rearranged sarcoma
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CDK4 binds with cyclin D
CDK4 regulation and activation
Contributed by Epitomics
Breast carcinoma
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Breast carcinoma
Liposarcoma - dedifferentiated (left), well differentiated (right)
Contributed by GenomeMe
Colon (normal), clone IHC402
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Colonic medullary carcinoma
Quality control of CDX2 staining
Contributed by Brandon Umphress, M.D. and Andrey Bychkov, M.D., Ph.D.
Porocarcinoma, ductal differentiation
Porocarcinoma with invasion into the dermis
Acrosyringeal unit
CEA expression in a poorly differentiated carcinoma
C cells in relation to thyroid follicle
Clustered C cells
Cases #195, #204, #108 and AFIP images
Bladder: paraganglioma
Kidney: carcinoid tumor
Parathyroid gland: ectopic tissue
Breast: neuroendocrine carcinomas
Thyroid: medullary carcinoma
Contributed by Jijgee Munkhdelger, M.D., Ph.D. and Andrey Bychkov, M.D., Ph.D.
Typical carcinoid immunoprofile
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Adrenal medulla:
pheochromocytoma
Breast: small cell carcinoma
Heart: metastatic
pheochromocytoma
(right side)
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Claudin5: normal and malignant (breast)
Claudin5: serous papillary adenocarcinoma (ovary)
Claudin7: synovial sarcoma
Claudin18: normal lung and stomach (splice variant 2)
Claudin18: PanIN (left), infiltrating ductal carcinoma (right)
Claudin18: carcinomas
of stomach, esophagus,
ovary (splice variant 2)
Contributed by GenomeMe
Lung (normal), clone IHC549
Contributed by Wei Chen, M.D., Ph.D. and Saba Shafi, M.D.
CRC biomarker testing algorithm
Contributed by Wei Chen, M.D., Ph.D.
MSI CRC histomorphology
MSI CRC with TILs
Normal staining (diffuse strong)
Normal staining (focal variability)
Absent / loss staining pattern
Tumor weaker than control
Cytoplasmic only staining
Contributed by Shrihari S. Kadkol, M.D., Ph.D.
MSI electropherogram
Contributed by Christian M. Schürch, M.D., Ph.D. and Kenneth A. Iczkowski, M.D.
Amyloidosis
Transthyretin amyloidosis
Distinguishing the correct color
Rotation to visualize Congo red
Optimized AL amyloid imaging
Breast amyloidosis
Case #388
Copper stain in liver, Wilson's disease
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Wilson's disease
Contributed by Nasir Ud Din, M.B.B.S.
Ewing sarcoma
Ewing sarcoma cyclin D1
Endometrial stromal sarcoma
Endometrial stromal sarcoma cyclin D1
Wilms tumor
Wilms tumor cyclin D1
Mantle cell lymphoma
Mantle cell lymphoma cyclin D1
Solid pseudopapillary neoplasm
Solid pseudopapillary neoplasm cyclin D1
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Synthesis and assembly of COX subunits
Maturation and insertion of COX into the respiratory chain
Contributed by Andrey Bychkov, M.D., Ph.D.
Lung SCC
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Breast carcinoma #1 is CK14+ (fig C)
Squamous cell carcinoma #1 oral (fig e/f)
Squamoid areas are CK14+ in urothelial carcinoma
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Breast myoepithelial hyperplasia (fig 1j)
Contributed by Leica Microsystems (Biosystems Division)
Colon (normal)
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Choroid plexus papilloma
Contributed by Andrey Bychkov, M.D., Ph.D.
CK19 expression in PTC
CK19: prominent membranous and cytoplasmic staining
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Squamous cell carcinoma, oral (fig B)
Stomach: complete intestinal metaplasia
Contributed by Leica Microsystems (Biosystems Division) and Andrey Bychkov, M.D., Ph.D.
Colon (normal):
CK20 (PW31) with
intense cytoplasmic
staining
Ovarian cancer
Urothelial carcinoma
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Colonic adenocarcinoma: #1-well differentiated; #2-poorly differentiated; #3-metastatic; #4-primary (fig G) and metastatic to lung (fig H)
PanIN lesions: CK20+ in grades 1, 2 and 3
Prostatic adenocarcinoma; #2 with positive staining of verumontanum
Tubular adenocarcinoma
Urothelial carcinoma, high grade
Contributed by Stephen J. Schultenover, M.D.
Thyroid anaplastic carcinoma
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Low grade bladder neoplasms
Contributed by Jijgee Munkhdelger, M.D., Ph.D., Andrey Bychkov, M.D., Ph.D. and Semir Vranić, M.D., Ph.D.
Breast tubular adenoma immunoprofile
Intraductal papilloma with DCIS immunoprofile
Intraductal papilloma with DCIS, CK5/6
Basal-like triple negative breast cancer
Apocrine metaplasia of the breast
Metastatic squamous cell carcinoma of the cervix
Apocrine carcinoma of the breast
Complex sclerosing lesion of the breast
Contributed by Kruti P. Maniar, M.D.
Cervical
squamocolumnar
junction
Cholangiocarcinoma
Colon
Liver portal tract
Lung adenocarcinoma
Ovarian seromucinous borderline tumor
Pancreas
Yolk sac tumor, glandular variant
Contributed by Andrey Bychkov, M.D., Ph.D., Eddie Fridman, M.D. (Case #194) and Leica Microsystems
Ovarian cancer
Urothelial carcinoma
Urethra: clear cell adenocarcinoma
Ureter (normal)
Contributed by Maria Tretiakova, M.D., Ph.D.
Normal kidney
Clear cell RCC
Clear cell papillary RCC
Papillary RCC, type1
Papillary RCC, type 2
Chromophobe RCC
Oncocytoma
Contributed by Leica Microsystems (Biosystems Division)
Colon (normal)
CK8 / 18 (5D3)
with intense cytoplasmic
and membranous staining
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Hepatocytes (residual)
are CK8+ in embryonal
sarcoma of liver
Liver disease (various)
Prostatic adenocarcinoma-top and benign prostate bottom
Squamous cell carcinoma-oral (fig C/D)
Contributed by Mieke R. Van Bockstal, M.D., Ph.D., Christine Galant, M.D., Ph.D. and Andrey Bychkov, M.D., Ph.D.
Serous carcinoma
Mammary spindle cell carcinoma
Mammary desmoid fibromatosis
Residual breast carcinoma
Ileal neuroendocrine tumor
Chordoma
Juvenile granulosa cell tumor
Sertoli-Leydig cell tumor
Uterine tumor resembling ovarian sex cord tumor (UTROSCT)
Normal liver
Normal lymph node
Gastric MALT lymphoma
Papillary thyroid cancer
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CK AE1 / AE3 highlights reactive mesothelial cells
Contributed by Kemal Kösemehmetoğlu, M.D.
Normal liver
Colon mucosa
Tonsil
Pancreas
Peritoneum
Dendritic cell staining in lymph node
Signet ring carcinoma of stomach
Signet ring carcinoma infiltrating omentum
Paget disease of nipple
Nipple duct adenoma
High grade neuroendocrine carcinoma
CAM 5.2 in Crooke cell adenoma
CAM 5.2 in sparsely granulated somatotroph adenoma
CAM 5.2 in sarcomatoid carcinoma
Inflammatory myofibroblastic tumor of the bladder
CAM 5.2 in epithelioid sarcoma (proximal variant)
CAM 5.2 in desmoplastic small round cell tumor
Case #78
Cardiac myxoma with glandular differentiation
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Anaplastic thyroid carcinoma is MNF116+ (fig A)
