Stains
MDR / CD243

Author: Kara Hamilton, M.S., Nat Pernick, M.D. (see Authors page)

Revised: 29 December 2015, last major update April 2010

Copyright: (c) 2002-2015, PathologyOutlines.com, Inc.

PubMed Search: CD243

Cite this page: MDR / CD243. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/cd243.html. Accessed December 8th, 2016.
Definition / General
  • "Detoxifying agent" that pumps toxins / drugs out of cells
  • Also transports steroid hormones and transports substances across blood-brain barrier
  • Also mediates the ATP-driven efflux of drugs from multidrug resistant cancer and HIV infected cells
Terminology
  • P glycoprotein is product of MDR1 gene
  • Also called MDR1 gene (multidrug resistance to cancer), P glycoprotein 170 (molecular weight is 170K), ABCB1, P-binding cassette subfamily B member 1
  • Antibody C219 binds to both ATP binding regions of P-glycoprotein and inhibits its ATPase activity and drug binding capacity
  • Widely used as a tumor marker, but cross reacts with HER2/CerbB2 (Proc Natl Acad Sci USA 1999;96:13679)
  • Antibody C494 detects an internal cellular epitope present only on the Mdr1 isoform of P-glycoprotein with strong plasma membrane staining patterns
    • It cross reacts with pyruvate carboxylase, an abundant mitochondrial enzyme (weak, homogeneous, cytoplasmic or granular patterns), but unlike C219, it does not cross react with Mdr3
  • Antibody JSB1 appears to have similar behavior as C494, as it cross reacts with pyruvate carboxlyase (J Histochem Cytochem 1995;43:1187)
Pathophysiology
Clinical Features
Uses by Pathologists
Micro Images

Images hosted on Other servers of Normal tissue:

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Duodenum, Fig.C/D

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Lung, Fig.1A

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Lung, Fig.D/E

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Retinal pigment epithelium



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Bladder

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Breast

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Hepatocellular carcinoma, globular intracytoplasmic stain

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Retinoblastoma, Fig.1&2

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Immunofluorescence



Positive Staining - Normal
Positive Staining - Disease