CD Markers
CD133

Reviewer: Nat Pernick, M.D. (see Authors page)

Revised: 31 December 2015, last major update June 2012

Copyright: (c) 2002-2015, PathologyOutlines.com, Inc.

PubMed Search: CD133[title]
Cite this page: CD133. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/cdmarkerscd133.html. Accessed December 4th, 2016.
Definition / General
  • Also known as Prominin 1, AC133
  • Transmembrane glycoprotein expressed on the hematopoietic stem cells, endothelial progenitors, and dermal-derived stem cells capable of differentiating into neural cells
Uses by Pathologists
  • Alternative to CD34 in selecting hematopoietic stem and progenitor cells for transplantation studies
  • Marker of cancer stem cells (cells with tumor initiating potential, Genes Chromosomes Cancer 2012;51:792
Clinical Features
Micro Images

Images hosted on other servers:

Glial tumors

Hepatocellular carcinoma and intrahepatic cholangiocarcinoma

Pancreas - normal

Stomach - gastric carcinoma


Colorectal carcinoma

Skin - nevus and melanoma

Skin - melanoma (childhood)

Positive Staining - Normal
  • Localized to microvilli and other plasma membrane protrusions
  • CD34 bright hematopoietic stem and progenitor cells (umbilical cord blood-derived CD133+ cells can differentiate into endothelial cells and induce new blood vessel growth, Front Biosci 2012;17:2247)
  • Progenitor cells within nephron (Expert Opin Ther Targets 2012;16:157)
  • Neural stem cells and other primitive cells such as retina
  • Villous and extravillous cytotrophoblast, syncytiotrophoblast
Positive Staining - Disease
  • Numerous malignancies
  • Brain tumors (CD133+ cells have characteristics of endothelial progenitor cells, Cancer Lett 2012;324:221)
Negative Staining
  • Adult epithelial tissue, villous stroma