CD Markers
CD34



Topic Completed: 1 May 2013

Minor changes: 16 November 2020

Copyright: 2002-2020, PathologyOutlines.com, Inc.

PubMed Search: CD34 [title] stain

Lauren N. Stuart, M.D., M.B.A.
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Cite this page: Stuart L.N. CD34. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/cdmarkerscd34.html. Accessed November 26th, 2020.
Definition / general
  • Commonly used marker of hematopoietic progenitor cells and endothelial cells (OMIM #142230)
Terminology
  • Also called hematopoietic progenitor cell antigen CD34
  • CD34+ stromal cells are called dendritic interstitial cells
Pathophysiology
  • Intercellular adhesion protein and cell surface glycoprotein; ligand is CD62L (L-selectin)
  • May mediate attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells, although specific function is unknown
  • CD34 staining defines adult hematopoietic stem cells, but CD34+ cells can also differentiate into neural cells (Exp Neurol 2006;197:399)
Clinical features
Interpretation
  • Membranous stain
  • Endothelium acts as a positive internal control
  • Staining represents presence of protein, not cross reactivity, despite wide range of tissues that are CD34+ (Am J Pathol 2000;156:21)
Uses by pathologists
Microscopic (histologic) images

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Images hosted on PathOut server:

Contributed by Andrey Bychkov, M.D., Ph.D.

GIST

Prominent vascular network

Capillary network


Case #190
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Storiform collagenoma



Case #182
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Neck tumor,
pseudoangiomatous
variant


Case #179
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Littoral cell angioma, spleen, CD34-


Case #151
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Leukemia vasculitis


Case #130
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Myeloid sarcoma, bone


Case #126
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PreB ALL, kidney


Case #84
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Sinonasal
hemangiopericytoma,
CD34-


Case #69
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Epithelioid sarcoma, CD34+


Case #23
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Epithelioid angiosarcoma, lung


 Other

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Hemangioendothelioma,
heart



Images hosted on other servers:

Normal:
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Fibroblastic cells (CD34+ in normal stomach, CD34- in intestinal gastric carcinoma)

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CD34+ stromal cells (normal stomach & colon, gastric adenocarcinoma)

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CD34+ vascular endothelial cells (normal and gastric adenocarcinoma)



 Gastrointestinal stromal tumor:
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Stomach GIST


Solitary fibrous tumor:
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Thigh



Vascular tumors:
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Angiosarcomas, liver

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Ovary

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Skin, scalp

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Epithelioid angiosarcoma, bone

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Hemangioendothelioma,
infantile
liver


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Epithelioid
hemangioendothelioma
of bone (left, fig 2B);
CNS (right, fig F)

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Hemangiomas, capillary type: endometrium (left, fig 2C); testis (right, fig 1B)

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Kaposi sarcoma



Other:
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Aggressive angiomyxoma: penis/scrotum (left); spermatic cord (right)

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Skin tumor, CD34+

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Breast carcinoma

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Hepatocellular carcinoma: tumor cells are CD34 negative, but intravascular tumor clusters are lined by CD34+ endothelial cells

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Various images

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Myofibroma, CD34-

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Neurothekeoma, CD34-

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Perineurioma, extraneural

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CD34+ fibroblastic cells


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Pseudoangiomatous hyperplasia (PASH) of breast: stroma is CD34+

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Solitary sclerotic fibroma, finger

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Spindle cell lipoma
has CD34+ stroma:
breast (left); salivary
glands (right, fig D)

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Superficial acral fibromyxoma, soft tissue, F2

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CD34 distinguishes displaced epithelium

Positive staining - normal
Positive staining - disease
Negative staining
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