CD Markers
CD34

Author: Lauren N. Stuart, M.D.

Revised: 13 June 2018, last major update May 2013

Copyright: (c) 2002-2018, PathologyOutlines.com, Inc.

PubMed Search: CD34 [title] stain

Cite this page: Stuart, L.N. CD34. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/cdmarkerscd34.html. Accessed July 23rd, 2018.
Definition / general
  • Commonly used marker of hematopoietic progenitor cells and endothelial cells (OMIM #142230)
Terminology
  • Also called hematopoietic progenitor cell antigen CD34
  • CD34+ stromal cells are called dendritic interstitial cells
Pathophysiology
  • Intercellular adhesion protein and cell surface glycoprotein; ligand is CD62L (L-selectin)
  • May mediate attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells, although specific function is unknown
  • CD34 staining defines adult hematopoietic stem cells, but CD34+ cells can also differentiate into neural cells (Exp Neurol 2006;197:399)
Clinical features
Interpretation
  • Membranous stain
  • Endothelium acts as a positive internal control
  • Staining represents presence of protein, not cross reactivity, despite wide range of tissues that are CD34+ (Am J Pathol 2000;156:21)
Uses by pathologists
Microscopic (histologic) images
Scroll to see all images

Images hosted on PathOut server

Contributed by
Andrey Bychkov, M.D., Ph.D.

GIST

Prominent vascular network

Capillary network


 Case of the Week
 #190

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Storiform collagenoma



 Case of the Week
 #182

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Neck tumor,
pseudoangiomatous
variant


 Case of the Week
 #179

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Littoral cell angioma, spleen, CD34-


 Case of the Week
 #151

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Leukemia vasculitis


 Case of the Week
 #130

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Myeloid sarcoma, bone


 Case of the Week
 #126

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PreB ALL, kidney


 Case of the Week
 #84

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Sinonasal
hemangiopericytoma,
CD34-


 Case of the Week
 #69

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Epithelioid sarcoma, CD34+


 Case of the Week
 #23

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Epithelioid angiosarcoma, lung



 Other

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Hemangioendothelioma,
heart



Images hosted on other servers:

Normal
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Blasts and megakaryocytes

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Fibroblastic cells (CD34+ in normal stomach, CD34- in intestinal gastric carcinoma)

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CD34+ stromal cells (normal stomach & colon, gastric adenocarcinoma)

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CD34+ vascular endothelial cells (normal and gastric adenocarcinoma)



 Gastrointestinal stromal tumor
 GIST
:

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GIST vs. SFT

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Stomach GIST



 Solitary fibrous tumor:

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Pleura (malignant tumor)

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Thigh



Vascular tumors:
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Angiosarcomas, liver

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Ovary

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Skin, scalp

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Epithelioid angiosarcoma, bone

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Hemangioendothelioma,
infantile
liver

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Epithelioid hemangioendothelioma of bone (left, fig 2B); CNS (right, fig F)

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Pleura

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Glomangioma of liver

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Hemangiomas, capillary type: endometrium (left, fig 2C); testis (right, fig 1B)

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Hemangiomas, cavernous type: lung (left, fig 1C); spermatic cord (right, fig B)

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Juvenile (FNA)

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Hemangiopericytoma,
CNS, CD34+

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Kaposi sarcoma



Other:
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Aggressive angiomyxoma: penis/scrotum (left); spermatic cord (right)

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Skin tumor, CD34+

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Angiomyofibroblastoma

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Breast carcinoma

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Chronic myelogenous leukemia in blast crisis

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Hepatocellular carcinoma: tumor cells are CD34 negative, but intravascular tumor clusters are lined by CD34+ endothelial cells

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Various images

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Myofibroma, CD34-

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Neurothekeoma, CD34-

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Perineurioma, extraneural

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Pseudoangiomatous hyperplasia (PASH) of breast: stroma is CD34+

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Solitary sclerotic fibroma, finger

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Spindle cell lipoma has CD34+ stroma: breast (left); salivary glands (right, fig D)

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Superficial acral fibromyxoma, soft tissue, F2

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CD34 distinguishes displaced epithelium

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CD34+ fibroblastic cells

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Fibrotic stroma, epithelial component

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Vascular leiomyoma, FA



Positive staining - normal
Positive staining - disease
Negative staining