Chemistry
Thyroid related
Thyroglobulin antibody

Author: Nat Pernick, M.D. (see Authors page)

Revised: 23 February 2016, last major update March 2011

Copyright: (c) 2002-2016, PathologyOutlines.com, Inc.

PubMed Search: Thyroglobulin antibody [title]
Table of Contents
Definition / General | Laboratory
Cite this page: Thyroglobulin antibody. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/chemistrythyroglobulnab.html. Accessed December 6th, 2016.
Definition / General
Laboratory
Indications
  • Marker of autoimmune thyroid disease (note: other thyroid antibodies are to TSH and thyroperoxidase)
  • Also ordered with serum thyroglobulin, since it may interfere with thyroglobulin assay
  • Modest correlation with presence of anti-thyroperoxidase antibodies (Autoimmunity 2006;39:497)

Methodology
  • Immunoassays now most common
  • Quantitative passive hemagglutination:
    • Formerly most common, uses chromic chloride hemagglutination or tanned red blood cells, 80% with Hashimoto's thyroiditis have titers > 1:1000
    • Also present, at lower titers, in Graves disease (60%), thyroid carcinoma (30%), pernicious anemia, Sjogren's syndrome, normals (3 - 18%)
  • Indirect immunofluorescence microscopy (uncommon):
    • Uses monkey thyroid gland tissue
    • Patterns are floccular, dull colloid spaces but bright peripheral fluorescence or diffuse bright uniform colloid staining in ground-glass pattern
    • 5 - 8% are false positives with positive immunofluorescence attributed to CA2 (second colloid antigen) but are negative for anti-thyroglobulin or anti-thyroperoxidase by other methods
  • Concordance between automated methods needs improvement (Clin Chim Acta 2007;376:88)

Interpretation
  • Present in patients with Hashimoto's thyroiditis, other autoimmune disease, also women with no clinical disease
  • Values < 4 .0 IU/ml are unlikely to interfere with thyroglobulin assay