Chemistry
Thyroid related
Thyroperoxidase antibody (Anti-TPO)

Author: Nat Pernick, M.D. (see Authors page)

Revised: 23 February 2016, last major update March 2011

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PubMed Search: Thyroperoxidase antibody [title]
Cite this page: Thyroperoxidase antibody (Anti-TPO). PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/chemistrythyroperoxidaseab.html. Accessed December 7th, 2016.
Definition / General
  • Associated with Hashimoto's thyroiditis (90%) and Graves disease (75%), although also present in women with no clinical disease
  • Antibody is directed against antigen within microsomal fraction of thyroid epithelial cells
Laboratory
  • Methodology:
    • ELISA, RIA, IRMA
    • Previously indirect immunofluorescence microscopy with substrate of air dried human or cryostat monkey tissue, looking for cytoplasmic but not nuclear staining of thyroid follicular cells
    • Concordance between automated methods needs improvement (Clin Chim Acta 2007;376:88)
Interpretation
  • Does not predict relapse of Graves disease after carbimazole treatment withdrawal (Thyroid 2006;16:1041)
Differential Diagnosis