Coagulation - Factor VIII inhibitor assay / Bethesda assay

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Coagulation

Coagulation laboratory tests

Factor VIII inhibitor assay / Bethesda assay

Reviewer: Jeremy Parsons, M.D. (see Reviewers page)
Revised: 21 June 2012, last major update June 2012
Copyright: (c) 2002-2012, PathologyOutlines.com, Inc.

General
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Methodology:
● Prepare serial dilutions of patient plasma in citrated saline from 1:1 to 1:160 (or higher)
● Mix each dilution with an equal volume of normal plasma containing a normal amount of coagulation factors, incubate for 2 hours, then perform factor VIII assay
● Titer of inhibitor is dilution that inhibits 50% of factor VIII in assay
● Can use porcine Factor VIII to assess cross-reactivity and assist in therapeutic decisions

Additional references
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● Am J Clin Pathol 2009;131:552

End of Coagulation > Coagulation laboratory tests > Factor VIII inhibitor assay / Bethesda assay

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