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Cytopathology
Palpation Guided Fine Needle Aspiration
Smear Making and Special Techniques for Recovering and Dividing Aspirate Material

Author: Joe D. Jakowski, M.D. (see Reviewers page)
Revised: 19 August 2013, last major update August 2013
Copyright: (c) 2013, PathologyOutlines.com, Inc.

Overview
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Type of FNA material obtained for smear making
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Solids: IMPORTANT: This firm solid material may be best triaged by picking it off the slide and submitting it for cell block or perhaps performing a touch preparation or a squash preparation instead of attempting to make a smear

Semisolid and special types of semisolids: Fluid:

Transferring FNA material from needle onto glass slide
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General: IMPORTANT: No smearing technique can correct clotting artifact

Techniques to ensure best results in transferring FNA material from needle to glass slide include:

Types of smear making methods
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General:

One step Smear Method
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General: Technique: Smear problems:

Two step Pull Method
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General: Technique: Smear problems:

Pop off Method
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General: Technique:

Simple Wick Concentration Method
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General: Technique:

Simple Incline Concentration Method
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General: Technique:

Two step Method for Concentration
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General: Technique:

Modified Two step Method for Concentration
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General: Technique:

Method(s) for dividing a single smear into several smears
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General: Technique #1: Technique #2:

Additional Special Techniques for Recovering Aspirate Material
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Needle Snap Technique: to recover material trapped in the hub of the needle Syringe Pop Technique: to recover material trapped in the tip or barrel of the syringe by rapidly and forcefully blowing it onto the surface of the glass slide
End of Cytopathology > Palpation Guided Fine Needle Aspiration > Smear Making and Special Techniques for Recovering and Dividing Aspirate Material


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