Kidney nontumor
Miscellaneous
Recommended special stains / IHC for kidney biopsies

Author: Carla Ellis M.D., M.S.

Revised: 18 July 2018, last major update January 2018

Copyright: (c) 2002-2018, PathologyOutlines.com, Inc.

PubMed Search: IHC kidney biopsies

Cite this page: Ellis. C Recommended special stains / IHC for kidney biopsies. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/kidneyspecialstainsforbiopsies.html. Accessed August 15th, 2018.
Routine special / immunohistochemical stains used to analyze native and transplant renal biopsies
  • Hematoxylin and eosin (H&E): normal kidney structure
  • C4d (Immunohistochemistry / Immunofluorescence) for transplant biopsies: useful in detection of antibody mediated rejection
  • Jones silver: highlights glomerular and basement membranes
  • Masson trichrome: highlights fibrosis (interstitial and glomerular)
  • Periodic acid Schiff (PAS): highlights glomerular and basement membranes
Additional immunohistochemical stains used to analyze native and transplant renal biopsies
  • Adenovirus immunohistochemistry
  • CMV immunohistochemistry
  • CD68: analogous use in other tissue to detect macrophage / histiocytic precursors
  • Collagen IV: useful in differentiating interstitial edema from interstitial fibrosis
  • Congo red: useful in detection of amyloidosis
  • COX2: useful in drug induced acute interstitial nephritis and drug induced minimal change disease
  • EBER: useful in detection of post transplant lymphoproliferative disorder (PTLD)
  • FN1: useful in fibronectin glomerulopathy
  • HSV immunohistochemistry
  • Myoglobin: useful in confirmation of rhabdomyolysis
  • NCCT: useful in detection of Sjogren syndrome
  • PAX2: useful in detection of Denys-Drash syndrome
  • Prealbumin: useful in detection of amyloidosis
  • SV40 Large T antigen (immunohistochemistry) for transplant biopsies: useful in detection of polyomavirus
  • von Kossa: detection of calcium deposits in calciphylaxis and malakoplakia