Lymphoma - B cell neoplasms
Molecular analysis - Fluorescent in-situ hybridization (FISH)
Reviewer: Nikhil Sangle, M.D., University of Utah and ARUP Laboratories (see Reviewers page)
Revised: 6 February 2012, last major update January 2011
Copyright: (c) 2001-2012, PathologyOutlines.com, Inc.
See also Molecular Pathology chapter
● Used to identify and count chromosomes / parts of chromosomes
● Detects and localizes the presence or absence of specific DNA sequences on chromosomes
● Interphase / metaphase preparation from formalin-fixed paraffin embedded tissue can be used
● Fluorescent probes bind to the area of interest on a chromosome; fluorescent microscopes are used to locate the probe
● Various lymphomas carry specific translocations; FISH can detect them rapidly and with high specificity, compared to conventional cytogenetics
CLL / SLL - trisomy 12, FISH shows three green signals in 2 cells on right side
PreB ALL with t(9;22)(q34;q11) - contributed by Dr. Julia Braza, Beth Israel Deaconess Medical Center, Boston
FISH shows one normal chromosome 16 and one with inv (16)
End of Lymphoma - B cell neoplasms > Lymph nodes > Molecular analysis - Fluorescent in-situ hybridization (FISH)
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