Lymphoma and plasma cell neoplasms
Lymph nodes
Molecular analysis - Fluorescent in situ hybridization (FISH)

Author: Nikhil Sangle, M.D. (see Authors page)

Revised: 3 April 2017, last major update January 2011

Copyright: (c) 2001-2017,, Inc.

PubMed Search: fluorescent in situ hybridization (FISH) lymph nodes

See also Molecular Pathology chapter
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Cite this page: Fluorescent in situ hybridization (FISH). website. Accessed August 21st, 2019.
Definition / general
  • Used to identify and count chromosomes / parts of chromosomes
  • Detects and localizes the presence or absence of specific DNA sequences on chromosomes
  • Interphase / metaphase preparation from formalin fixed paraffin embedded tissue can be used
  • Fluorescent probes bind to the area of interest on a chromosome; fluorescent microscopes are used to locate the probe
  • Various lymphomas carry specific translocations; FISH can detect them rapidly and with high specificity, compared to conventional cytogenetics
Microscopic (histologic) images

Images hosted on PathOut server:

PreB ALL with t(9;22)(q34;q11),
contributed by Dr. Julia Braza,
Beth Israel Deaconess Medical Center, Boston

Images hosted on other servers:

FISH shows one normal chromosome 16 and one with inv (16)

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