Molecular markers
Molecular cytology competency assessment
Techniques in molecular pathology

Author: Zubair W. Baloch, M.D., Ph.D. (see Authors page)

Revised: 7 February 2018, last major update February 2014

Copyright: (c) 2002-2018,, Inc.

PubMed Search: Techniques in molecular pathology [title] review[ptyp]

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Cite this page: Baloch, Z.W. Techniques in molecular pathology. website. Accessed December 18th, 2018.
Medical knowledge
  1. Basics
    • Understand isolation and quantitation of DNA and RNA from cells
    • Understand enzymatic digestion of DNA
    • Understand the basis of electrophoretic separation of nucleic acids
    • Understand the utility of specific probes and labeling of DNA and RNA
  2. Amplification of DNA and RNA
    • Understand and define polymerase chain reaction (PCR)
    • Understand non PCR based methods for amplification
    • Understand allele specific PCR
  3. Understand nucleic acid detection methods
  4. Describe other techniques for mutation screening and detection
  5. Understand computer based analyses
Practice based learning and systems based practice
  1. Develop a familiarity with principles and techniques used in the design and validation of molecular patient tests