Molecular markers
DNA purification
Anion exchange chromatography

Author: Rodney E. Shackelford, D.O., Ph.D. (see Authors page)

Revised: 29 May 2018, last major update April 2012

Copyright: (c) 2008-2018, PathologyOutlines.com, Inc.

PubMed Search: Anion exchange chromatography[TI] DNA purification

Table of Contents
Definition / general
Cite this page: Shackelford, R.E. Anion exchange chromatography. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/moleculardnapuranionexchange.html. Accessed July 22nd, 2018.
Definition / general
  • Subtype of ion exchange chromatography (Wikipedia: Ion Chromatography [Accessed 29 May 2018], Tosoh Bioscience: Ion Exchange [Accessed 29 May 2018])
  • Rapid and efficient method of nucleic acid purification which removes lipids, proteins and other cellular constituents in one rapid step
  • Uses a positively charged solid resin column that captures negatively charged nucleic acids
  • Prior to use, the column is equilibrated with a buffer containing anions and the crude nucleic acid preparation (usually a cell lysate) is added to the column
  • Negatively charged nucleic acids are preferentially retained in the column while other cellular constituents are washed out, allowing one step purification
  • After several washes to remove unwanted cellular constituents, the nucleic acids are removed with a high salt, low pH wash
  • Commonly, the elution profiles of RNA and DNA overlap and other steps must be undertaken if pure RNA or DNA is required
  • Diethylaminoethyl (DEAE) covalently linked to sepharose (a polysaccharide polymer) is used in anion exchange chromatography