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Molecular Pathology

DNA Purification

Commercial DNA Extraction Machines


Author: Rodney E. Shackelford, D.O., Ph.D. (see Reviewers page)
Revised: 14 April 2012, last major update April 2012
Copyright: (c) 2008-2012, PathologyOutlines.com, Inc.

Definition
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● Many DNA extraction machines are commercially available, allowing high-throughput simultaneous processing of many samples
● Most commercial extractors use an initial lysis step to free nucleic acids, followed by binding to a solid phase such as magnetic glass beads or a silica membrane
● The solid phase is then extensively washed to remove soluble contaminants, and the nucleic acids are removed with heating, high-salt buffers, or other methods to lower their affinity for the solid matrix
● Separation of the solid-phase bound nucleic acids is often facilitated by centrifugation, or “pull down” of magnetic beads in a magnetic field
● Commercial DNA extraction machines were first introduced in the early 1990’s, and now are vastly more efficient, with higher throughput volumes, better extraction and final purity, and lower cost per extraction

End of Molecular Pathology > DNA Purification > Commercial DNA Extraction Machines


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