Table of ContentsDefinition / general
Cite this page: Shackelford R. Commercial DNA extraction machines. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/moleculardnapurcommercialDNA.html. Accessed June 16th, 2019.
Definition / general
- Many DNA extraction machines are commercially available, allowing high throughput simultaneous processing of many samples
- Most commercial extractors use an initial lysis step to free nucleic acids, followed by binding to a solid phase such as magnetic glass beads or a silica membrane
- Solid phase is then extensively washed to remove soluble contaminants and the nucleic acids are removed with heating, high salt buffers or other methods to lower their affinity for the solid matrix
- Separation of the solid phase bound nucleic acids is often facilitated by centrifugation or pull down of magnetic beads in a magnetic field
- Commercial DNA extraction machines were first introduced in the early 1990s and now are vastly more efficient, with higher throughput volumes, better extraction and final purity and lower cost per extraction