Table of ContentsDefinition / general
Cite this page: Shackelford, R.E. PCR inhibitors. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/moleculardnapurpcrinhibitors.html. Accessed October 15th, 2018.
Definition / general
- PCR inhibitors are commonly encountered; removing these containments is vital to insure good PCR amplification
- Chemical structure of PCR inhibitors and mechanism(s) of inhibition show enormous variation
- Inhibitors include excess salts such as KCl and NaCl, detergents / hydrophobic chemicals (phenol, SDS, chloroform, sodium deocyholate and sarkosyl), inappropriate heating, loss of reagents required for PCR (such as Mg++, Taq polymerase denaturation, etc.), alcohols (ethanol and isopropyl alcohol), heparin, bromophenol blue, agarose, inosine, humic acids, deoxyuridine, excess nucleic acids in the amplification reaction, various polysaccharides (OpenWetWare: PCR Inhibitors [Accessed 29 May 2018])
- Since inhibitors can be either hydrophilic or hydrophobic (or both), DNA purification purification protocols usually involve subjecting the crude nucleic acid mix to both hydrophilic and hydrophobic washing steps