Author: Rodney E. Shackelford, D.O., Ph.D. (see Reviewers page)
Revised: 14 April 2012, last major update April 2012
Copyright: (c) 2008-2011, PathologyOutlines.com, Inc.
● PCR inhibitors are commonly encountered; removing these containments is vital to insure good PCR amplification
● The chemical structure of PCR inhibitors and mechanism(s) of inhibition show enormous variation
● Inhibitors include excess salts such as KCl and NaCl, detergents/hydrophobic chemicals (phenol, SDS, chloroform, sodium deocyholate, and sarkosyl), inappropriate heating, loss of reagents required for PCR (such as Mg++, Taq polymerase denaturation, etc), alcohols (ethanol and isopropyl alcohol), heparin, bromophenol blue, agarose, inosine, humic acids, deoxyuridine, excess nucleic acids in the amplification reaction, various polysaccharides (OpenWetWare)
● Since inhibitors can be either hydrophilic or hydrophobic (or both), DNA purification purification protocols usually involve subjecting the crude nucleic acid mix to both hydrophilic and hydrophobic washing steps
End of Molecular Pathology > DNA Purification > PCR Inhibitors
This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must be interpreted in the context of a patient's clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician.
All information on this website is protected by copyright of PathologyOutlines.com, Inc. Information from third parties may also be protected by copyright. Please contact us at copyrightPathOut@gmail.com with any questions (click here for other contact information).