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Molecular Pathology

DNA sequencing

Automated Reading, Automated Parallel Sample Loading and Bioinformatics


Author: Rodney E. Shackelford, D.O., Ph.D., Tulane University (see Reviewers page)
Revised: 13 February 2011, last major update February 2011
Copyright: (c) 2008-2011, PathologyOutlines.com, Inc.

General
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● Other innovations that improved sequencing technology were the implementation of parallel processing, automated parallel sample loading and automated sequence analysis
● With parallel processing, multiple samples are loaded and separated via capillary electrophoresis simultaneously, allowing a vast increase in the amount of material that can be analyzed at once (i.e. high-throughput analysis)
● Current automated parallel sample loading allows many samples to be quickly and accurately loaded for capillary electrophoresis, bypassing the slow process of manually loading each sample as in slab gel electrophoresis
● Similarly, the development of automated sequence analysis and the computer programs required to facilitate accurate analyses has allowed faster and more efficient data interpretation
● The development of bioinformatics has been essential in allowing the sequencing and analysis of large DNA sequences, such as the human genome. Although earlier sequence analysis programs often had unsatisfactory base-calling algorithms which required further human analysis, later analysis programs overcame these flaws and could analyze DNA sequences with a high degree of certainty
● The overall effect of the above innovations was to increase the read-length, speed, efficiency, accuracy, and throughput of DNA sequencing, while lowering sequencing costs

End of Molecular Pathology > DNA sequencing > Automated Reading, Automated Parallel Sample Loading and Bioinformatics


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