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Capillary electrophoresis (CAE)




Topic Completed: 1 February 2011

Revised: 15 February 2019

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PubMed Search: Capillary electrophoresis CAE

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Definition / general
Cite this page: Shackelford R. Capillary electrophoresis (CAE). PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/molecularpathdnaseqcapillary.html. Accessed March 20th, 2019.
Definition / general
  • Capillary electrophoresis (CAE) uses narrow bore capillaries with internal diameters below 100 mm
  • The high surface area to volume of the tubes increases heat dissipation, allowing a higher electrical field to be used for electrophoresis, leading to an 8 to 10 fold faster separation time than slab gel electrophoresis
  • The use of improve matrices, such as non cross linked polymer solutions, has allowed ~1000 bp to be read efficiently and accurately in as little as 2 hours
  • The introduction of capillary electrophoresis was a milestone that allowed the sequencing of the human genome (Nucleic Acids Res 2007;35:6227)
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