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Molecular Pathology

DNA sequencing

Capillary Electrophoresis (CAE)


Author: Rodney E. Shackelford, D.O., Ph.D., Tulane University (see Reviewers page)
Revised: 18 September 2012, last major update February 2011
Copyright: (c) 2008-2011, PathologyOutlines.com, Inc.

General
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● Capillary electrophoresis (CAE) uses narrow-bore capillaries with internal diameters below 100 mm
● The high surface area to volume of the tubes increases heat dissipation, allowing a higher electrical field to be used for electrophoresis, leading to an 8 to 10-fold faster separation time than slab gel electrophoresis
● The use of improve matrices, such as non-cross-linked polymer solutions, has allowed ~ 1000 bp to be read efficiently and accurately in as little as 2 hours
● The introduction of capillary electrophoresis was a milestone that allowed the sequencing of the human genome (Nucleic Acids Res 2007;35:6227)

End of Molecular Pathology > DNA sequencing > Capillary Electrophoresis (CAE)


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