Table of ContentsDefinition / general
Cite this page: Shackelford, R.E. Capillary electrophoresis (CAE). PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/molecularpathdnaseqcapillary.html. Accessed December 15th, 2018.
Definition / general
- Capillary electrophoresis (CAE) uses narrow bore capillaries with internal diameters below 100 mm
- The high surface area to volume of the tubes increases heat dissipation, allowing a higher electrical field to be used for electrophoresis, leading to an 8 to 10 fold faster separation time than slab gel electrophoresis
- The use of improve matrices, such as non cross linked polymer solutions, has allowed ~1000 bp to be read efficiently and accurately in as little as 2 hours
- The introduction of capillary electrophoresis was a milestone that allowed the sequencing of the human genome (Nucleic Acids Res 2007;35:6227)