Capillary electrophoresis (CAE)

Molecular markers
DNA sequencing
Capillary electrophoresis (CAE)

Author: Rodney E. Shackelford, D.O., Ph.D. (see Authors page)

Revised: 30 May 2018, last major update February 2011

Copyright: (c) 2008-2018, PathologyOutlines.com, Inc.

PubMed Search: Capillary electrophoresis CAE

Table of Contents

Definition / general

Cite this page: Shackelford, R.E. Capillary electrophoresis (CAE). PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/molecularpathdnaseqcapillary.html. Accessed December 15th, 2018.

Definition / general

Capillary electrophoresis (CAE) uses narrow bore capillaries with internal diameters below 100 mm
The high surface area to volume of the tubes increases heat dissipation, allowing a higher electrical field to be used for electrophoresis, leading to an 8 to 10 fold faster separation time than slab gel electrophoresis
The use of improve matrices, such as non cross linked polymer solutions, has allowed ~1000 bp to be read efficiently and accurately in as little as 2 hours
The introduction of capillary electrophoresis was a milestone that allowed the sequencing of the human genome (Nucleic Acids Res 2007;35:6227)