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Molecular Pathology

DNA sequencing


Author: Rodney E. Shackelford, D.O., Ph.D., Tulane University (see Reviewers page)
Revised: 13 February 2011, last major update February 2011
Copyright: (c) 2008-2011, PathologyOutlines.com, Inc.


● Several next generation DNA sequencing techniques employ nanotechnology to increase sequencing speed
● Pacific Biosciences (website) is working on a DNA sequencing platform to produce 100 Gb of sequence data/hour, or enough sequencing capacity and speed to give one-fold coverage to the diploid human genome in 4 minutes; it is based on Single Molecule Real Time, where natural DNA synthesis by DNA polymerase is observed and measured by interrogating thousands of nanometer scale aperture chambers in 100 nm metal film (called zero-mode waveguides) deposited on a silicon dioxide substrate
● Each aperture provides a nanophotonic visualization chamber with a detection volume of 20 zeptoliters (10 -21 liters)
● This small volume allows the detection of a single molecule in a background of thousands of labeled nucleotides
● To date, this technology achives read-lengths of 1500 bp, with a read-rate of 10 bases/sec

End of Molecular Pathology > DNA sequencing > Nanotechnology

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