Molecular markers
DNA sequencing
Nanotechnology

Author: Rodney E. Shackelford, D.O., Ph.D. (see Authors page)

Revised: 30 May 2018, last major update February 2011

Copyright: (c) 2008-2018, PathologyOutlines.com, Inc.

PubMed Search: Nanotechnology[TI] DNA sequencing

Table of Contents
Definition / general
Cite this page: Shackelford, R.E. Nanotechnology. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/molecularpathdnaseqnano.html. Accessed August 16th, 2018.
Definition / general
  • Several next generation DNA sequencing techniques employ nanotechnology to increase sequencing speed
  • Pacific Biosciences is working on a DNA sequencing platform to produce 100 Gb of sequence data/hour or enough sequencing capacity and speed to give one fold coverage to the diploid human genome in 4 minutes; it is based on Single Molecule Real Time, where natural DNA synthesis by DNA polymerase is observed and measured by interrogating thousands of nanometer scale aperture chambers in 100 nm metal film (called zero mode waveguides) deposited on a silicon dioxide substrate
  • Each aperture provides a nanophotonic visualization chamber with a detection volume of 20 zeptoliters (10-21 liters)
  • This small volume allows the detection of a single molecule in a background of thousands of labeled nucleotides
  • To date, this technology achives read lengths of 1500 bp, with a read rate of 10 bases/sec