Home   Chapter Home   Jobs   Conferences   Fellowships   Books


Molecular Pathology

DNA sequencing

Real-time single-molecule DNA sequence determination

Author: Rodney E. Shackelford, D.O., Ph.D., Tulane University (see Reviewers page)
Revised: 13 February 2011, last major update February 2011
Copyright: (c) 2008-2011, PathologyOutlines.com, Inc.


● VisiGen Biotechnologies has engineered a DNA polymerase which acts as a real-time sensor of modified nucleotides
● This method uses DNA synthesis, employing a DNA polymerase with a donor fluorescent dye close to the polymerase active site where nucleotides to be incorporated are selected
● The four dNTPs are also modified, with each having a different acceptor dye compatible with the polymerase donor dye
● At dNTP incorporation, the donor dye transfers fluorescent resonant energy to the acceptor dye, resulting in measurable light release
● The four different acceptor dyes give off different wave length emissions, allowing the specific dNTP incorporated to be determined
● The advantage of this system is that it can sequence DNA at the same rate at the polymerase incorporates dNTPs - several hundred moieties per second, with read-lengths of around 1 kb, longer than possible with any current platform
● This technology may eventually allow incredible sequencing feats, such a sequencing the entire human genome in one day for ~$1,000 (N Biotechnol 2009;25:195)

End of Molecular Pathology > DNA sequencing > Real-time single-molecule DNA sequence determination

This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must be interpreted in the context of a patient's clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician.

All information on this website is protected by copyright of PathologyOutlines.com, Inc. Information from third parties may also be protected by copyright. Please contact us at copyrightPathOut@gmail.com with any questions (click here for other contact information).