Molecular markers
Microarray variations
Antibody microarrays

Author: Rodney E. Shackelford, D.O., Ph.D. (see Authors page)

Revised: 1 June 2018, last major update July 2010

Copyright: (c) 2008-2018, PathologyOutlines.com, Inc.

PubMed Search: Antibody microarrays[TI]

Cite this page: Shackelford, R.E. Antibody microarrays. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/molecularpathmicroarrayantibody.html. Accessed July 22nd, 2018.
Definition / general
  • Different groups of antibodies may be attached to a solid matrix, allowing the microarray to be performed on solutions of proteins, such as cell lysates, urine, plasma or cerebrospinal fluid
  • This assay can be performed with many different variations
Methodology
  • One common method uses the standard enzyme linked immunosorbent assays (ELISA) protocol:
    1. Specific protein I solution is bound by a solid matrix attached antibody
    2. Washing
    3. Binding of a second detection antibody which binds the specific protein
    4. Washing
    5. Addition of a second antibody which has an attached reporter (a fluorophore or horse radish peroxidase [HRP] commonly)
    6. Washing
    7. Specific protein binding is detected via the addition of a HRP substrate or exposure to light wavelengths which cause the fluorophore to emit