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Molecular Pathology

Microarrays

Variations - Antibody microarrays

Author: Rodney E. Shackelford, DO, Ph.D. (see Reviewers page)

Revised: 8 September 2012, last major update July 2010

Copyright: (c) 2008-2012, PathologyOutlines.com, Inc.

General

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● Different groups of antibodies may be attached to a solid matrix, allowing the microarray to be performed on solutions of proteins, such as cell lysates, urine, plasma or cerebrospinal fluid

● This assay can be performed with many different variations

Methodology

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One common method uses the standard enzyme-linked immunosorbent assays (ELISA) protocol:

(1) a specific protein I solution is bound by a solid matrix-attached antibody

(2) washing

(3) binding of a second detection antibody which binds the specific protein

(4) washing

(5) addition of a second antibody which has an attached reporter (a fluorophore or horse radish peroxidase [HRP] commonly)

(6) washing

(7) specific protein binding is detected via the addition of a HRP substrate or exposure to light wavelengths which cause the fluorophore to emit

End of Molecular Pathology > Microarray > Variations > Antibody microarrays

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