Molecular - Microarray - Bead based microarray

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Molecular Pathology

Microarray

Variations - Bead based microarray

Author: Rodney E. Shackelford, DO, Ph.D. (see Reviewers page)

Revised: 3 July 2010, last major update July 2010

General

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● Recently, microscopic polystyrene or latex beads have been employed as the solid support in microarray

● Beads can carry two or more labels in different ratios creating different bead types, allowing a more complex analysis than microarray performed on a two dimensional matrix

● Additionally, up to 100 different fluorophores can be used to label 100 different bead-attached probes

● The beads may be placed in a microtitre plate, commonly allowing 96 different samples to be analyzed in parallel

● Following mixing with labeled target cDNAs, hybridization and washing, the beads can by analyzed flow cytometry

● The advantage of this system is that hybridization takes place in solution (instead of a two-dimensional surface), often giving a higher specificity for closely related target sequences

End of Molecular Pathology > Microarray > Variations > Bead based microarray

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