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Molecular Pathology

Microarray

Variations - Bead based microarray

 

Author: Rodney E. Shackelford, DO, Ph.D. (see Reviewers page)

Revised: 3 July 2010, last major update July 2010

Copyright: (c) 2008-2010, PathologyOutlines.com, Inc.

 

General

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● Recently, microscopic polystyrene or latex beads have been employed as the solid support in microarray

Beads can carry two or more labels in different ratios creating different bead types, allowing a more complex analysis than microarray performed on a two dimensional matrix

Additionally, up to 100 different fluorophores can be used to label 100 different bead-attached probes

The beads may be placed in a microtitre plate, commonly allowing 96 different samples to be analyzed in parallel

Following mixing with labeled target cDNAs, hybridization and washing, the beads can by analyzed flow cytometry

The advantage of this system is that hybridization takes place in solution (instead of a two-dimensional surface), often giving a higher specificity for closely related target sequences

 

End of Molecular Pathology > Microarray > Variations > Bead based microarray

 

 

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