Molecular markers
Microarray variations
Bead based microarray

Topic Completed: 1 July 2010

Revised: 15 February 2019

Copyright: 2008-2018,, Inc.

PubMed Search: Bead based microarray

Rodney E. Shackelford, D.O., Ph.D.
Page views in 2018: 13
Page views in 2019 to date: 14
Table of Contents
Definition / general
Cite this page: Shackelford R. Bead based microarray. website. Accessed September 24th, 2019.
Definition / general
  • Recently, microscopic polystyrene or latex beads have been employed as the solid support in microarray
  • Beads can carry two or more labels in different ratios creating different bead types, allowing a more complex analysis than microarray performed on a two dimensional matrix
  • Additionally, up to 100 different fluorophores can be used to label 100 different bead attached probes
  • Beads may be placed in a microtitre plate, commonly allowing 96 different samples to be analyzed in parallel
  • Following mixing with labeled target cDNAs, hybridization and washing, the beads can by analyzed flow cytometry
  • Advantage of this system is that hybridization takes place in solution (instead of a two dimensional surface), often giving a higher specificity for closely related target sequences
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