Table of ContentsDefinition / general
Cite this page: Shackelford R. PCR thermocycling machines. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/molecularpathpcrthermocycling.html. Accessed May 27th, 2020.
Definition / general
- Early PCR protocols required that each reaction be manually transferred to water baths of different temperatures at each temperature change
- This problem was eliminated with the development of PCR thermocyclers, which rapidly change reaction temperatures without the need for manual transfers
- The first machine for this purpose, "Mr. Cycle," was developed by Cetus Corporation
- Since then, many different thermocyclers have been developed; most can be programmed to allow any desired temperature and time for each PCR cycle
- More recent PCR machines can analyze multiple parallel real time PCR reactions and analyze the data via various computer algorithms