Molecular - PCR - PCR thermocycling machines

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Molecular Pathology

Polymerase Chain Reaction

PCR thermocycling machines

Author: Rodney E. Shackelford, D.O., Ph.D. (see Reviewers page)

Revised: 22 September 2012, last major update July 2010

General

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● Early PCR protocols required that each reaction be manually transferred to water baths of different temperatures at each temperature change

● This problem was eliminated with the development of PCR thermocyclers, which rapidly change reaction temperatures without the need for manual transfers

● The first machine for this purpose, “Mr. Cycle”, was developed by Cetus Corporation

● Since then, many different thermocyclers have been developed; most can be programmed to allow any desired temperature and time for each PCR cycle

● More recent PCR machines can analyze multiple parallel real-time PCR reactions and analyze the data via various computer algorithms

End of Molecular Pathology > Polymerase Chain Reaction > PCR thermocycling machines

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