Home   Chapter Home   Jobs   Conferences   Fellowships   Books





Molecular Pathology

Polymerase Chain Reaction

PCR thermocycling machines


Author: Rodney E. Shackelford, D.O., Ph.D. (see Reviewers page)

Revised: 22 September 2012, last major update July 2010

Copyright: (c) 2008-2010, PathologyOutlines.com, Inc.




● Early PCR protocols required that each reaction be manually transferred to water baths of different temperatures at each temperature change

● This problem was eliminated with the development of PCR thermocyclers, which rapidly change reaction temperatures without the need for manual transfers

● The first machine for this purpose, “Mr. Cycle”, was developed by Cetus Corporation

● Since then, many different thermocyclers have been developed; most can be programmed to allow any desired temperature and time for each PCR cycle

● More recent PCR machines can analyze multiple parallel real-time PCR reactions and analyze the data via various computer algorithms


End of Molecular Pathology > Polymerase Chain Reaction > PCR thermocycling machines



This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must also be interpreted in the context of a patient's clinical data using reasonable medical judgment.  This website should not be used as a substitute for the advice of a licensed physician.


All information on this website is protected by copyright of PathologyOutlines.com, Inc.  Information from third parties may also be protected by copyright.  Please contact us at copyrightPathOut@gmail.com with any questions (click here for other contact information).