Polymerase Chain Reaction
PCR thermocycling machines
Author: Rodney E. Shackelford, D.O., Ph.D. (see Reviewers page)
Revised: 22 September 2012, last major update July 2010
Copyright: (c) 2008-2010, PathologyOutlines.com, Inc.
● Early PCR protocols required that each reaction be manually transferred to water baths of different temperatures at each temperature change
● This problem was eliminated with the development of PCR thermocyclers, which rapidly change reaction temperatures without the need for manual transfers
● The first machine for this purpose, “Mr. Cycle”, was developed by Cetus Corporation
● Since then, many different thermocyclers have been developed; most can be programmed to allow any desired temperature and time for each PCR cycle
● More recent PCR machines can analyze multiple parallel real-time PCR reactions and analyze the data via various computer algorithms
End of Molecular Pathology > Polymerase Chain Reaction > PCR thermocycling machines
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