Stains - 7 AAD

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Stains

7 AAD / 7-amino-actinomycin D

Reviewer: Nat Pernick, M.D., PathologyOutlines.com, Inc. (see Reviewers page)
Revised: 6 April 2011, last major update April 2011
Copyright: (c) 2002-2011, PathologyOutlines.com, Inc.

General
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● Fluorescent dye that binds DNA and is excited by a 488 nm laser, commonly used in flow cytometry
● Intact cells exclude 7 AAD, but dead cells allow 7 AAD entry, which binds to DNA (Wikipedia)

Diagrams
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7 AAD fluorescence

Uses
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● Used in flow cytometry to reduce non-specific staining by eliminating 7 AAD positive cells (dead cells) from further analysis

Positive staining - normal
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● Dead cells: allow entry of dye, which binds DNA and fluoresces with 488 nm laser commonly used in flow cytometry

Negative staining
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● Viable cells (do not allow entry of dye into cell)

End of Stains > 7 AAD

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