Stains
7 AAD / 7-amino-actinomycin D


Topic Completed: 1 July 2013

Revised: 4 March 2019

Copyright: 2003-2018, PathologyOutlines.com, Inc.

PubMed Search: 7 AAD

Nat Pernick, M.D.
Page views in 2018: 48
Page views in 2019 to date: 34
Cite this page: Pernick N. 7 AAD / 7-amino-actinomycin D. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stains7aad.html. Accessed July 17th, 2019.
Definition / general
  • Fluorescent dye that binds DNA and is excited by a 488 nm laser, commonly used in flow cytometry
  • Intact cells exclude 7 AAD, but dead or apoptotic cells that have lost membrane intergrity allow 7 AAD entry, which binds to DNA (Wikipedia, Nat Protoc 2007;2:187)
Diagrams / tables

7 AAD fluorescence

Uses by pathologists
  • Used in flow cytometry to reduce non-specific staining by eliminating 7 AAD positive cells (dead cells) from further analysis
  • Can use to exclude dead sperm (Andrologia 2010;42:20)
Positive staining - normal
  • Dead cells: allow entry of dye, which binds DNA and fluoresces with 488 nm laser commonly used in flow cytometry
Negative staining
  • Viable cells (do not allow entry of dye into cell)
Back to top