Author: Kara Hamilton, M.S., Nat Pernick, M.D. (see Authors page)

Revised: 2 August 2018, last major update July 2009

Copyright: (c) 2002-2018,, Inc.

PubMed Search: HE4 stain

Page views in 2018: 76
Page views in 2019 to date: 4
Cite this page: Hamilton, K., Pernick, N. HE4. website. Accessed January 18th, 2019.
Definition / general
  • Antibody against Human Epididymis protein 4, a protease inhibitor
  • Also called WAP5 and WFDC2 (WAP four-disulfide core domain 2)
Uses by pathologists
Microscopic (histologic) images

Images hosted on other servers:

Respiratory tract and salivary glands

Cystic fibrosis of lung

Major and minor glands (Fig B, E)

Pulmonary neoplasms

Ovarian carcinoma - various subtypes

Positive staining - normal
  • Breast (female)
  • Colonic mucosa
  • Epididymis (Biol Reprod 1991;45:350) and vas deferens
  • Female genital tract (endocervix, endometrium, fallopian tube, ovary)
  • Renal tubules
  • Respiratory epithelium
  • Salivary glands
Positive staining - disease
Negative staining
  • Most nonovarian carcinomas