Stains - Acridine orange

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Stains

Acridine orange

Reviewer: Nat Pernick, M.D., PathologyOutlines.com, Inc. (see Reviewers page)
Revised: 24 April 2011, last major update April 2011
Copyright: (c) 2002-2011, PathologyOutlines.com, Inc.

Uses
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(1) To identify small numbers of bacteria and microorganisms under fluorescent light, including mycobacteria (Indian J Pathol Microbiol 2010;53:894) and malaria parasites (Ann Trop Med Parasitol 2002;96:643)
(2) In fertility testing, is part of sperm chromatin structural assay, which reflects sperm chromatin denaturation (J Assist Reprod Genet 2009;26:591)
(3) Part of photodynamic therapy to treat musculoskeletal sarcoma (J Bone Joint Surg Br 2010;92:760)
(4) To identify engulfed apoptotic cells, because it fluoresences upon engulfment

Pathophysiology
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● Cell-permeable, interacts with DNA by intercalation and with RNA by electrostatic attraction

Micro images
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Fluoresent staining of mycobacteria

Fluorescent staining of trypanosomes and some platelets

Staining of chromatin in sperm of man with history of recurrent spontaneous abortion in partner

End of Stains > Acridine orange

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