Author: Nat Pernick, M.D. (see Authors page)

Revised: 30 October 2015, last major update January 2012

Copyright: (c) 2003-2015,, Inc.

PubMed Search: BRAF[title]
  • Serine / threonine specific protein kinase, part of the RAS/MAPK pathway regulating the growth, maturation, proliferation, survival and apoptosis of cells
  • Abbreviation for v-raf murine sarcoma viral oncogene homolog B1
  • Gene commonly mutated in papillary thyroid carcinoma and melanoma
  • V600E mutation (valine at residue 600 replaced by glutamic acid) renders BRAF constitutively active and increases its kinase activity (Nature 2002;417:949)

MAPK signaling pathway

Clinical Features
  • The drug Vemurafenib / Zelboraf┬«, used to treat melanoma, interrupts the BRAF/MEK/ERK pathway, only when BRAF has the V600E mutation (valine replaced by glutamic acid)
Uses by Pathologists
Micro Images

Prevalence of BRAF mutations in different histologic variants of thyroid papillary carcinoma

Negative Stains
Molecular / Cytogenetics Description
Molecular / Cytogenetics Images

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BRAF melting curves

Images hosted on

Chromatograms in Spitz nevus (left) and diffuse sclerosing papillary thyroid carcinoma (right)

Mass spectrometry traces for BRAF mutation