Reviewer: Nat Pernick, M.D. (see Reviewers page)
Revised: 18 October 2013, last major update June 2005
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PubMed Search: von Kossa method Alizarin red S Azan stain
- With routine staining, calcium forms a blue-black lake with hematoxylin, usually with sharp edges
- von Kossa method: silver is substituted for calcium in calcium salts; light or a photographic developer turns the silver black; only stains calcium bound to an anion such as phosphate or carbonate; most useful when large amounts of phosphates and carbonates are present, as with bone
- Alizarin red S forms an orange-red lake with calcium at a pH of 4.2; works best with small amounts of calcium such as in Michaelis-Gutman bodies; this method is used on Dupont ACA analyzer to measure serum calcium photometrically
- Azan stain used to distinguish osteoid from mineralized bone.
Malakoplakia of bladder (von Kossa calcium stain)
End of Stains > Calcium stains
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