Stains & molecular markers
Cytokeratin 14 (CK14, K14)


Topic Completed: 1 October 2013

Minor changes: 5 February 2020

Copyright: 2003-2020, PathologyOutlines.com, Inc.

PubMed Search: CK14[title]

Nat Pernick, M.D.
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Cite this page: Pernick N. Cytokeratin 14 (CK14, K14). PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsck14.html. Accessed August 7th, 2020.
Definition / general
Uses by pathologists
  • Distinguish parathyroid oxyphil adenoma (CK14+) from carcinoma (CK14-, Am J Surg Pathol 2002;26:344)
  • Distinguish breast papilloma (stronger and more diffuse CK14 staining) from papillary DCIS (Am J Surg Pathol 2005;29:625)
  • Distinguish sinonasal squamous cell carcinoma (poorly differentiated or nonkeratinizing, both CK14+) from sinonasal undifferentiated carcinoma or nasopharyngeal carcinoma (CK14-, Am J Surg Pathol 2002;26:1597)
Positive staining - normal
  • Basal keratinocytes in stratified epithelium (various tissue/organs)
  • Hair follicles (Br J Dermatol 2004;150:860)
  • Myoepithelial cells (breast and salivary gland)
  • Thyroid oncocytes
Positive staining - not malignant
  • Breast papilloma (see above)
  • Odontogenic neoplasms (Oral Dis 2003;9:1)
  • Parathyroid oxyphil adenoma (see above)
  • Pseudoepitheliomatous hyperplasia-spinous and superficial layers of oral mucosa with paracoccidioidomycosis (Med Mycol 2006;44:399)
  • Renal and other oncocytoma (Histopathology 2001;39:455)
  • Thymoma
  • Trichoblastoma
Positive staining - malignant
Negative staining
  • Normal oral mucosa, most renal cell carcinomas
Microscopic (histologic) images

Contributed by GenomeMe

Colon (normal), clone IHC555, 10x, 20x, 40x (respectively)


Contributed by Andrey Bychkov, M.D., Ph.D.

Lung SCC



Images hosted on other servers:

Breast carcinoma #1 is CK14+ (fig C)

Squamous cell carcinoma #1 oral (fig e/f)

Squamoid areas are CK14+ in urothelial carcinoma

Additional references
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