Stains - Cytokeratin 34 beta E12

Home Chapter Home Jobs Conferences Fellowships Books

Stains

Cytokeratin 34 beta E12

Authors: Kara Hamilton, M.S., Nat Pernick, M.D., PathologyOutlines.com, Inc.

Revised: 15 April 2010, last major update - June 2009

Definition

=========================================================================

● High molecular weight keratin relatively specific for prostate basal cells

● Reacts to CK1, CK5, CK10 and CK14 and possibly other keratins

Terminology

=========================================================================

● Also called CK903, high molecular weight keratin

Uses

=========================================================================

(1) Prostate:

● Stains prostatic basal cells (even after destaining H&E slides, Hum Path 2000;31:1155), whose presence rules out usual type prostatic adenocarcinoma

● Is useful in biopsies-AJCP 2004;121:99 and even after hormonal therapy-Hum Path 2007;38:332)

● Note that patchy staining may rarely occur in carcinoma (AJSP 2002;26:1151) and negative staining does not predict carcinoma in small foci of atypical glands (Hum Path 2004;35:43)

● Triple cocktail with p63 and AMACR is more sensitive/specific than 34 beta E12 alone (AJCP 2007;127:248)

● Distinguishes high grade PIN (intact or fragmented basal cell layer) from adenocarcinoma (Mod Path 2004;17:360)

● Distinguishes cribriform basal cell hyperplasia from cribriform PIN (AJSP 2002;26:237)

● Typically does not stain prostatic adenocarcinoma cells, but does stain prostatic adenoid cystic/basal carcinoma (AJSP 2003;27:1523) and tumors with squamous differentiation (AJSP 2004;28:651)

(2) Breast intraductal lesions:

● Distinguishes lobular intraepithelial neoplasia (cytoplasmic staining, often with distinct nuclear pattern, J Histochem Cytochem 2003;51:1527) or florid ductal hyperplasia without atypia (intense staining, AJSP 1999;23:1048), from DCIS / ductal intraepithelial neoplasia (usually negative, Hum Path 2002;33:620)

(3) Urothelial lesions:

● Distinguishes dysplasia (basal staining only) from carcinoma in situ (stains all urothelial layers, Hum Path 2000;31:745)

● Staining pattern predicts recurrence in low grade papillary urothelial neoplasms (Cancer 2003;97:1876)

● Is a marker of urothelial origin when used with a panel (AJSP 2003;27:1)

Positive stains - normal

=========================================================================

● Prostate basal cells, skin (J Exp Clin Cancer Res 2003;22:441)

● Thyroid solid cell nests (AJSP 2006;30:994)

Positive stains – not carcinoma

=========================================================================

● Amyloid deposits associated with squamous cell carcinoma (SCC) and dysplasia in the head and neck (Pathol Int 2003;53:265)

● Breast ductal hyperplasia and lobular intraductal neoplasia (references above)

● Placental site nodules (Pathology 1999;31:328)

● Prostatic basal cell hyperplasia (Hum Path 2003;34:462)

● Thymoma (high grade, Rom J Morphol Embryol 1999-2004;45:153)

Positive stains - carcinoma

=========================================================================

● Breast metaplastic carcinoma, clear cell carcinoma of gynecologic tract (Int J Gynecol Pathol 2001;20:252)

● Endocervical and endometrial carcinoma (Int J Gynecol Pathol 2002;21:11)

● Kidney collecting duct carcinoma (Zhonghua Zhong Liu Za Zhi 2001;23:162)

● Lung-non small cell carcinoma (44%, AJSP 2003;27:1)

● Ovary (Int J Gynecol Pathol 2001;20:155)

● Squamous cell carcinoma (classic and basaloid-Hum Path 1998;29:609)

● Thyroid CASTLE tumor (AJSP 2006;30:994)

● Thyroid papillary carcinoma (Appl Immunohistochem Mol Morphol 2000;8:42)

● Urothelial carcinoma (80%, AJSP 2003;27:1) and carcinoma in situ (references above)

Negative stains

=========================================================================

● Paget’s disease of vulva (Zhonghua Fu Chan Ke Za Zhi 1999;34:156)

● Prostatic secretory and stromal cells

● Prostatic adenocarcinoma of usual type (references above)

● Renal clear cell carcinoma (Int J Gynecol Pathol 2001;20:155)

● Renal papillary carcinoma (Zhonghua Zhong Liu Za Zhi 2005;27:102)

Micro images

=========================================================================

breast-lobular intraepithelial neoplasia

kidney-collecting duct carcinoma with signet ring features

skin-squamous cell carcinoma-acantholytic type

thyroid anaplastic carcinoma #1; #2

prostate - normal; atrophy with pseudoinfiltrative growth pattern; basal cell hyperplasia; post-atrophic hyperplasia; sclerosing adenosis; atypical adenomatous hyperplasia #1; #2; high grade PIN #1-tufting pattern with 34BE12 staining; #2-micropapillary pattern; #3-flat pattern; #4-with foamy cytoplasm; #5-with adenocarcinoma; staining without and with antigen retrieval; prostatic adenocarcinoma is negative with occasional positive benign glands #1; #2; #3; #4; adenoid basal cell tumor; urothelial type adenocarcinoma with mucin production is focally 34BE12+

Additional references

=========================================================================

Sources for Cytokeratin 34 beta E12 (advertisements): Covance

End of Stains > Cytokeratin 34 beta E12

This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must also be interpreted in the context of a patient's clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician.

All information on this website is protected by Copyright, (c) 2001-2009, PathologyOutlines.com, Inc. Information from third parties may also be protected by copyright. Please contact us at copyrightPathOut@gmail.com with any questions.