Pathology Outlines

Stains
deltaNp63 (p40)

Author: Nat Pernick, M.D. (see Authors page)

Revised: 2 December 2015, last major update January 2012

Copyright: (c) 2002-2015, PathologyOutlines.com, Inc.

PubMed Search: deltaNp63 [title]

Table of Contents

Cite this page: deltaNp63. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsdeltaNp63.html. Accessed July 12th, 2017.

Definition / general

One of 10 p63 isoforms (J Virol 2007;81:11569), important to classify non small cell lung cancer into adenocarcinoma (p40-) versus squamous cell carcinoma (p40+), which determines chemotherapy regimen
Truncated, nontransactivating isoform highly specific for squamous / basal cells

Terminology

Also called ΔNp63, p40
Different from these p40s:
- Eukaryotic initiation factor 3 (eIF3-p40) (J Virol 2007;81:11569)
- Interleukin 12 / cytokine p40 (J Biol Chem 2011;286:29806) or its antibodies used to treat psoriasis (Semin Cutan Med Surg 2010;29:48)
- Lysosomal membrane p40 (Biochem J 2008;414:431)
- Phagocyte NADPH oxidase subunit p40 phox (Blood 2009;114:3309)
- Klebsiella pneumoniae Toll-like receptor2 ligand P40 (J Immunother 2009;32:875)
- Lactobacillus casei BL23 (J Mol Microbiol Biotechnol 2010;19:231)
- Mycoplasma p40 (Microbiology 2011;157:473)
- Saccharomyces cerevisiae p40 / ARPC1 (J Biol Chem 2010;285:8481)

Clinical features

Mutations cause ectrodactyly-ectodermal dysplasia-cleft syndrome, ankyloblepharon-ectodermal dysplasia-clefting syndrome, nonsyndromic split-hand/split-foot malformation (J Cell Sci 2011;124:2200)

Uses by pathologists

Classify non-small cell lung carcinoma on small biopsies or cell blocks:
- Adenocarcinoma (ΔNp63- or <2% of cells with weak staining)
- Squamous cell carcinoma (ΔNp63+ [strong] and >50% of cells, TTF1-)
- Adenosquamous (ΔNp63 strong and less than 50%, J Thorac Oncol 2012;7:281, Am J Surg Pathol 2012;36:895)
May help classify large cell lung carcinoma as part of panel (Am J Clin Pathol 2011;136:773)

Microscopic (histologic) images

Images hosted on Other servers:

Hidradenocarcinoma, metastatic to lymph node

Lung, various tumors

Oral preneoplastic lesions (OPL)

Nature images:

Pancreas, various images

Positive staining - normal

Breast myoepithelial cells (Am J Surg Pathol 2001;25:1054)
Cytotrophoblast (Am J Surg Pathol 2004;28:1177)
Prostate myoepithelial cells

Positive staining - disease

Adamantinoma-like Ewing family tumor (Am J Surg Pathol 2013;37:772)
Esophagus: precancerous and cancerous squamous lesions (Hum Pathol 2003;34:850)
Lung: squamous cell carcinomas (100%)
Salivary gland: adenoid cystic, mucoepidermoid, myoepithelial carcinomas (Hum Pathol 2005;36:821)

Negative staining

Normal pancreatic ducts (Mod Pathol 2005;18:1193)
Lung: adenocarcinoma (5% are positive, but in <5% of cells, Mod Pathol 2012;25:405)
Lymphoma: large cell
Pancreas: squamous differentiation (Mod Pathol 2005;18:1193)