Giemsa stain

Author: Nat Pernick, M.D.

Revised: 1 June 2018, last major update October 2013

Copyright: (c) 2003-2018,, Inc.

PubMed Search: Giemsa stain
Page views in 2018: 2,019
Page views in 2019 to date: 123
Cite this page: Pernick, N. Giemsa stain. website. Accessed January 18th, 2019.
Definition / general
  • As a hematology stain, works best with alcohol fixed smears
  • As a histology stain, detects mast cells and microorganisms, such as Giardia or Helicobacter
  • A "Romanowsky-type" stain, composed of mixtures of methylene blue, azure, and eosin compounds
  • Methylene blue is a metachromatic stain, meaning that some tissue components (mast cell granules, cartilage, mucin, amyloid) stain purple and not blue
Microscopic (histologic) images

Images hosted on PathOut server:

Contributed by Andrey Bychkov, M.D., Ph.D., Kameda Medical Center

H. pylori