Stains
Giemsa stain

Author: Nat Pernick, M.D. (see Authors page)

Revised: 17 December 2015, last major update October 2013

Copyright: (c) 2003-2015, PathologyOutlines.com, Inc.

PubMed Search: Giemsa stain
Table of Contents
Definition / General
Definition / General
  • As a hematology stain, works best with alcohol fixed smears
  • As a histology stain, detects mast cells and microorganisms, such as Giardia or Helicobacter
  • A "Romanowsky-type" stain, composed of mixtures of methylene blue, azure, and eosin compounds
  • Methylene blue is a metachromatic stain, meaning that some tissue components (mast cell granules, cartilage, mucin, amyloid) stain purple and not blue