Stains
Giemsa stain

Author: Nat Pernick, M.D.

Revised: 10 May 2018, last major update October 2013

Copyright: (c) 2003-2018, PathologyOutlines.com, Inc.

PubMed Search: Giemsa stain
Cite this page: Pernick, N. Giemsa stain. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsgiemsa.html. Accessed May 21st, 2018.
Definition / general
  • As a hematology stain, works best with alcohol fixed smears
  • As a histology stain, detects mast cells and microorganisms, such as Giardia or Helicobacter
  • A "Romanowsky-type" stain, composed of mixtures of methylene blue, azure, and eosin compounds
  • Methylene blue is a metachromatic stain, meaning that some tissue components (mast cell granules, cartilage, mucin, amyloid) stain purple and not blue
Microscopic (histologic) images

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Contributed by Andrey Bychkov, M.D., Ph.D.

H. pylori