Stains
H3K27me3


Topic Completed: 29 January 2019

Revised: 03 January 2019

Copyright: (c) 2002-2019, PathologyOutlines.com, Inc.

PubMed Search: H3K27me3 [title]

Brendan C. Dickson, M.D., M.Sc.
Page views in 2018: 65
Page views in 2019 to date: 1,360
Cite this page: Nawar N, Dickson BC. H3K27me3. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsh3k27me3.html. Accessed October 18th, 2019.
Definition / general
Essential features
Pathophysiology
Interpretation
  • H3K27me3 is constitutively expressed and intact expression is denoted by the presence of diffuse nuclear staining; loss of staining may be complete or heterogeneous (mosaic) (Mod Pathol 2016;29:4)
  • Interpretation is context dependent
  • An eccentric intranuclear dot reflects the inactivated X chromosome in normal tissues (Histopathology 2016;69:702)
Uses by pathologists
Prognostic factors
Microscopic (histologic) images

Images hosted on PathOut server:

Contributed by Brendan Dickson, M.D.

Malignant peripheral nerve sheath tumor

Malignant triton tumor with rhabdomyoblastic differentiation

Schwannoma

Positive staining - normal
  • Normal tissue contains intact nuclear H3K27me3 expression
  • Presence of an eccentric intranuclear dot reflects the inactivated X chromosome in normal tissues (Histopathology 2016;69:702)
Positive staining - disease
  • Most tumor tissues contain intact nuclear H3K27me3 expression
Negative staining - normal
  • To date, no normal tissues have been reported to consistently lack H3K27me3 expression
Negative staining - disease
Board review question #1
    A tumor is biopsied in a patient with a remote history of cancer. It shows a cellular spindle cell neoplasm with a herringbone pattern. Which of the following neoplasms is most likely to exhibit loss of H3K27me3?

  1. Cellular schwannoma
  2. Epithelioid malignant peripheral nerve sheath tumor
  3. Neurofibroma
  4. Radiation associated malignant peripheral nerve sheath tumor
Board review answer #1
D. Radiation associated malignant peripheral nerve sheath tumor. Complete loss of H3K27me3 occurs in > 90% of radiation associated malignant peripheral nerve sheath tumor. It is intact in the vast majority of cases of epithelioid malignant peripheral nerve sheath tumor, schwannoma and neurofibroma.

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