Stains
MDM2

Author: Nat Pernick, M.D. (see Authors page)

Revised: 5 November 2015, last major update March 2013

Copyright: (c) 2002-2015, PathologyOutlines.com, Inc.

PubMed Search: MDM2 [title]

Definition / General
  • Murine Double Minute 2
  • Protein that inhibits p53; gene is at 12q13-15
Clinical Features
  • Amplified in well-differentiated liposarcoma and dedifferentiated liposarcoma (amplification detected by comparative genomic hybridization, qualitative PCR, FISH; MDM2 immunostain correlates with gene amplification
  • MDM2 T309G polymorphism might be a risk factor for leukemia among Asians but not Caucasians (Leuk Lymphoma 2012;53:2245)
Uses by Pathologists
  • Distinguish well-differentiated liposarcoma (positive, FISH is 90% sensitive, Mod Pathol 2012;25:1384) from benign adipose tumors (negative); FISH is more sensitive than immunostains on needle biopsies (Mod Pathol 2010;23:1301)
  • Distinguish dedifferentiated liposarcoma (positive) from poorly differentiated sarcomas (negative)
  • Distinguish low-grade osteosarcoma (positive) from benign fibrous and fibro-osseous lesions (negative, Mod Pathol 2011;24:624)
  • MDM2 and CDK4 coexpression in high grade osteosarcoma suggests progression from low grade osteosarcoma (Am J Surg Pathol 2012;36:423)
  • CDK4 is more specific but less sensitive than MDM2 (Am J Surg Pathol 2005;29:1340)
Micro Images

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MPNST



Nature images:

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Liposarcoma

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Liposarcoma, inflammatory

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Osteosarcoma, low grade



Molecular / Cytogenetics Images

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Dedifferentiated liposarcoma



Nature images:

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MDM2 amplification



Positive Staining - Disease
Negative Staining