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Stains
PAS (Periodic Acid-Schiff)

Reviewer: Nat Pernick, M.D. (see Reviewers page)
Revised: 12 September 2013, last major update June 2005
Copyright: (c) 2003-2013, PathologyOutlines.com, Inc.
PubMed Search: periodic acid schiff[title]

General
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Theory
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Uses
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Positive staining (normal)
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Positive staining (disease)
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Negative staining
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Micro images
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Bacteria:

Atypical mycobacteria

Rhodococcus is PAS+ in colonic histiocytes (far right)

Rhodococcus is PAS+ in pulmonary histiocytes (PASD+)

Tropheryma whipplei in histiocytes in hippocampus (fig 3)


Breast cytology:

PASD+ malignant tumors

PASD “not actually positive” benign tumors


CNS:

Acquired hepatocellular degeneration (with Luxol fast blue)

Secretory meningioma PAS+ inclusions


Colon:

Malakoplakia (fig 3: PAS, 4: calcium stain)


Cornea:

Fuch’s dystrophy


Fungi:

Blastomyces

Candida of esophagus

Lung

Tongue

Coccidioidis immitis of tongue (fig 4)

Cryptococcal meningitis

Histoplasmosis of colon in HIV+ woman (fig 3)

Microsporidia

Mucor of stomach


Heart:

Glycogen storage disease

Glycogen storage disease (removed by diastase)


Kidney:

Normal

Alport’s syndrome

Alport’s syndrome (fetal glomerulus)

Amyloidosis

Chronic pyelonephritis

Dense deposit disease

Fabry’s disease

Focal segmental glomerulosclerosis

Henoch Schoenlein purpura

IgA nephropathy

Immunotactoid glomerulonephritis

Membranoproliferative glomerulonephritis type I

With crescent


With doubling of capillary wall

Nephronophthisis

Nodular glomerulosclerosis

SLE

Thrombotic microangiopathy


Liver:

Alpha-1-antitrypsin deficiency

Hepatitis A with confluent necrosis


Lung:

Pneumocystis pneumonia

Pulmonary alveolar proteinosis: globules (B/D) are PAS+; A/C are H&E

Surfactant in hyaline membrane disease


Parasites:

Acanthamoeba histolytica (colon)

Acanthamoeba histolytica (cornea)

Acanthamoeba histolytica (brain)

Echinococcus (fig 2/3) in bone


Parotid gland:

Normal


Skeletal muscle:

Increased glycogen (PAS+, PASD-) in desmin related myopathy

PAS+ diastase sensitive material in McArdle’s disease


Skin:

Fabry's disease


Small intestine:

Normal (fig 2), microvillous inclusion disease (fig 3/4)


Tumors:

Acinar cell carcinoma of pancreas

Adenocarcinoma of colon

Alveolar soft parts sarcoma in cervix

Clear cell acanthoma

Clear cell carcinoma of ovary

Cylindroma

Eccrine spiradenoma

Endolymphatic sac papillary tumor of ear

Ewing’s sarcoma


Glycogen rich carcinoma of breast
fig 1/2: infiltrating nests of atypical cells
with abundant clear cytoplasm
3: PAS+ granules
4: PAS staining is removed by diastase

Glycogen rich urothelial carcinoma

Granular cell tumor

Juxtaglomerular cell tumor of kidney

Kaposi’s sarcoma


Lymphoplasmacytic lymphoma

Mucinous cystadenoma of ovary

Paget’s disease-penis

Secretory carcinoma of breast

Secretory meningioma

Tubulocystic carcinoma of kidney

Virtual slides
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Cryptococcus of brain

Additional references
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End of Stains > PAS (Periodic Acid-Schiff)


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