Stains
Phosphohistone H3

Author: Nat Pernick, M.D. (see Authors page)

Revised: 24 July 2018, last major update June 2005

Copyright: (c) 2002-2018, PathologyOutlines.com, Inc.

PubMed Search: Phosphohistone H3 [title]

Page views in 2018: 839
Page views in 2019 to date: 86
Cite this page: Pernick, N. Phosphohistone H3. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsphosphohistoneh3.html. Accessed January 22nd, 2019.
Definition / general
  • Core histone protein that is major constituent of chromatin; marker of cells in late G2 and M phase
Interpretation
  • Nuclear stain
  • Count phosphohistone H3+ objects (nuclei and mitoses) in 10 adjacent fields with 40x objective, in invasive epithelial areas with highest phosphohistone H3 staining
  • Ignore nuclei with fine granular staining
Uses by pathologists
Microscopic (histologic) images

Images hosted on other servers:
Missing Image

Normal colonic mucosa

Missing Image

Ki67, phosphohistone H3 and survivin

Missing Image

Grade 3 breast carcinoma

Missing Image

Thick cutaneous melanomas

Positive staining - disease
  • Mitotic activity