Establishing the spatial / cellular context of gene expression and validating NGS gene discoveries using
RNA in situ Hybridization
Pronin et al. used RNA-seq to sequence the entire
transcriptome of the mouse cornea and identified
~10,000 actively transcribed genes. Among them
there were 194 GPCRs, of which 96 were putative
olfactory receptors (Olfrs). They went on to validate
the 20 Olfrs by RT-PCR and RNA in situ hybridization
(ISH) via RNAscope® technology.
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understand new molecular techniques to:
Expression of Olfr558 in vessels of the choroid and retina
(Pronin et al., 2014). In situ hybridization of the mouse choroid
slices with Olfr558 probe (red dots). Nuclei staining is DAPI (blue
dots).