Establishing the spatial / cellular context of gene expression and validating NGS gene discoveries using RNA in situ Hybridization
Pronin et al. used RNA-seq to sequence the entire
transcriptome of the mouse cornea and identified
~10,000 actively transcribed genes. Among them
there were 194 GPCRs, of which 96 were putative
olfactory receptors (Olfrs). They went on to validate
the 20 Olfrs by RT-PCR and RNA in situ hybridization
(ISH) via RNAscope® technology.
Join a webinar on Tues, Jan 20th, 2015 at 11 am (Pacific US Time), 2 pm (Eastern US Time) and understand new molecular techniques to:
Expression of Olfr558 in vessels of the choroid and retina (Pronin et al., 2014). In situ hybridization of the mouse choroid slices with Olfr558 probe (red dots). Nuclei staining is DAPI (blue dots).