Stains and Molecular Markers by Subspecialty

Breast stains: androgen receptor calponin CK5 CK7 E-cadherin estrogen receptor/ER GATA3 GCDFP-15 HER2 breast Ki67 mammaglobin p40 p63 p120 catenin progesterone receptor/PR SMMHC / smooth muscle myosin heavy chain

Derm stains:
Melanocytic tumors: HMB45 MART1 / MelanA MITF S100 SOX10 tyrosinase
Melanoma mutations: ARID2 BAP1 BRAF GNAQ HRAS CD117 / KIT NF1 NRAS PTEN
Keratinocytic and adnexal neoplasms: CEA CK5 CK6 CK7 EMA p40 p63 pancytokeratin
Spindle cell neoplasms: CD31 CD34 desmin ERG Factor XIIIa p63 S100
Specialized markers: BAP1 BRAF MMR proteins: MLH1 MSH2 MSH6 PMS2

GI stains:
Esophagus: CMV GMS
Stomach: CD117 / KIT chromogranin DOG1 gastrin (pending) Helicobacter pylori Ki67 synaptophysin
Small intestine: CD117 / KIT chromogranin DOG1 Ki67 synaptophysin
Colon: CDX2 CMV chromogranin Ki67 synaptophysin
Pancreas: IgG4 Ki67 SMAD4 / DPC4
Liver: arginase1 chromogranin CMV HepPar1 iron PAS reticulin serum amyloid A / SAA trichrome

GU / Adrenal stains:
Adrenal: calretinin chromogranin GATA3 Inhibin MelanA S100 synaptophysin
Bladder / urothelial: CK7 CK20 GATA3 p40 p63 Uroplakin II (pending)
Kidney - common use: AE1/AE3 AMACR CAIX CAM5.2 CD10 CD117 / KIT CK7 EMA PAX8 RCC Vimentin
Kidney - specific scenarios: BRAF Cathepsin K CD57 chromogranin fumarate hydratase (pending-JJW) FLI1 HMB45 HMWCK INI1 MelanA S100A1 (pending) SDHB SMA synaptophysin TFE3 WT1
Prostate: androgen receptor AMACR CK5 CK5/6 34betaE12 / HMWCK / high molecular weight NKX3.1 p63 Prostein (P501S) PSA PSAP PSMA
Testis: AFP CD30 CD117 / KIT CK7 D2-40 GATA3 Glypican 3 hCG inhibin OCT3/4 PLAP SALL4

Gyn stains:
Cervix: BCL2 CDX2 CEA CK7 ER GATA3 HIK1086 Ki67 MUC6 NapsinA p16 p40 p53 p63 PAX8 PR vimentin
Endometrium: AMACR ARID1A hCG cyclin E E-cadherin ER GATA3 HNF-1B (pending) HPL IMP3 (pending) inhibin MEL-CAM/CD146 NapsinA p16 p53 p57 PAX2 PAX8 PLAP PR PTEN
Fallopian tube: BCL2 Ki67 p53
Ovary: AE1/AE3 ARID1A beta catenin hCG calretinin CDX2 CK7 CK20 CD30 EMA ER FOXL2 Glypican 3 inhibin MUC1 MUC2 MUC5A MUC6 NapsinA OCT3/4 p16 p53 PAX8 PTEN reticulin SALL4 SATB2 WT1
Peritoneum: BerEP4 calretinin CD10 D2-40 PAX8 WT1
Uterus-mesenchymal: Alcian blue ALK caldesmon CD10 Cyclin D1 desmin EMA HMB45 IFITM1 (pending) Ki67 MelanA MEL-CAM/CD146 myogenin myoglobin p16 p53 smooth muscle actin
Vulva and vagina: CD34 CK17 desmin HMGA2 (pending) Ki67 p16 p53

Head and neck stains:
Ear: cytokeratin AE1/AE3 GFAP GMS (special) IgG (pending) IgG4 S100 synaptophysin
Gnathic (maxilla / mandible): beta catenin BRAF calretinin CD56 cytokeratin AE1/AE3 Ki67 LEF-1 (pending) MUC4 S100 SOX2 synaptophysin
Larynx: CK5 CK7 cytokeratin AE1/AE3 GMS (special) p40 p63 S100
Nasal / sinonasal / nasopharynx: beta catenin CD45 CD99 CDX2 CK7 CK20 cytokeratin AE1/AE3 desmin EBER1 (in situ hybridization) INI1 NUT p16 p40 S100 SMA SOX10 synaptophysin
Salivary: androgen receptor beta catenin calponin CK5 CK7 cytokeratin AE1/AE3 GATA3 IgG (pending) IgG4 Ki67 LEF-1 (pending) mucicarmine (special) MYB p40 p63 PLAG1 S100 SMA
Thyroid main: calcitonin CK19 Galectin3 HBME thyroglobulin TTF1
Thyroid additional: AE1/AE3 BCL2 beta catenin BRAF CD5 CD117 / KIT CEA chromogranin Congo Red Ki67 mammaglobin p53 p63 PAS PAX8 PPARγ PTEN

Medical renal stains: recommended special stains / IHC

Pulmonary stains:
Adenocarcinoma: CK7 NapsinA TTF1
Squamous cell carcinoma: CK5/6
Small cell carcinoma: chromogranin synaptophysin TTF1
Mesothelioma: CAIX CK5/6 calretinin D2-40 WT1



Alphabetical listing

IHC basics IHC procedure common panels tissue of origin/unknown primary enzyme cytochemistry

2-succino-cysteine (pending) 3 beta 5-hmC (pending) 7 AAD 11A1 14-3-3sigma 15-PGDH 45M1

ABCC2 A beta 42 abl acid fast acid phosphatase ACSM1 acridine orange actin-general actin-alpha cardiac actin-alpha smooth muscle actin-muscle specific adenovirus Adipophilin (pending) AE1/AE3 (cytokeratin) AF4 AGR2 AKT albumin alcian blue ALK alkaline phosphatase ALL1 alpha-1-antichymotrypsin alpha-1-antitrypsin alpha-fetoprotein (AFP) alpha-lactalbumin alpha-synuclein AMACR AML1 AMY1A amylase (pending) amyloid beta / APP androgen receptor annexin A1 (pending) annexin A10 (pending) AP2beta APC API2-MALT1 Apolipoprotein D argentaffin arginase1 ARID1A ARID2 asbestos ATM ATDX (ATRX) auramine-rhodamine

B72.3 BAP1 BCL1 BCL10 (pending) BCL2 BCL6 Bcl-xL bcr-abl Beclin1 BerEP4 beta-2-microglobulin beta catenin BG7 BG8 Bielschowsky biotin blood group antigens BMP BOB1 bombesin brachyury BRAF BRAF (melanoma) BRCA1 BRCA2 BUB1B Butyrate esterase (4-methylumbelliferyl butyrate) (pending)

C3 (pending) C4d (pending) CD117 / KIT CDH17 (pending) c-MET CA 19-9 CA125 cadherins calcitonin calcium caldesmon calponin calretinin CAMTA1 CAM5.2 carbonic anhydrase IX / CAIX caspases cathepsin B cathepsin K CCND1 CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 CCR9 * CD Markers (separate chapter) * CD117 / KIT CDC2 CDK4 CDX2 CEA ceramide chaperones chloroacetate esterase chromaffin chromogranin claudins claudin1 claudin5 claudin6 claudin7 claudin18 clusterin CMV collagen collagen II collagen IV collagen XVII Congo Red COX COX2 Crystal Violet (pending) CXCL13 (pending) CXCL16 CXCR1 CXCR2 CXCR3 CXCR5 cyclins Cyclin D1 cyfra 21-1 (pending)

Cytokeratins: general CK1 CK2 CK3 CK4 CK5 CK5/6 CK6 CK7 CK8 CK8/18 (pending) CK9 CK10 CK11 CK12 CK13 CK14 CK15 CK16 CK17 CK18 CK19 CK20 CK21 CK22 CK23 CK24 34betaE12 / HMWCK / high molecular weight 35betaH11 AE1 AE3 AE1/AE3 CAM5.2 KL-1 MNF116 OSCAR

D2-40 DBA-44 DCC deltaNp63 desmogleins desmin dicer DOG1 DOPA reaction DPC4 DR6 Dupan-2 (pending) dynactin 1 Dystrophin (pending)

E1AF E2A E-cadherin EBER1 EGFR EKH4 keratin (pending) eIF-4F elastic fibers elongin EMA EMB (pending) endothelin-1 EpCAM Epstein-Barr virus (EBV) ERCC1 ERG estrogen receptor/ER ets1 ETV1 ETV6-NTRK3 EWSR1 EZH2

Factor VIII Factor XIIIa Fas/CD95 Fas ligand/CD178 fascin ferritin FGFR3 FHIT filaggrin FLI1 FLT3 FMC7 FN1 (pending) Follicular dendritic cell secreted protein (FDCSP) (pending) Fontana-Masson FOXA1 FOXJ1 (pending) FOXL2 FOXP3 fumarate hydratase (pending)

Galectin3 Gastrin (pending) GATA3 GCDFP-15 GFAP Giemsa GITR glucagon (pending) GLUT1 GLUT4 glutamine synthetase (pending) glycophorin A glycosoaminoglycans glypican 3 GMS GNAQ GNAS gram stain granzyme B granzyme M (pending) Grimelius

H3K27me3 Hales colloidal iron HAM56 hamartin Hansel HBME hCG HE4 HEG1 (pending) Helicobacter pylori Hemoglobin A (pending) HepPar1 HER2 breast HER2 nonbreast HES1 HGAL HHF35 HHV8 HIF1a (pending) HIK1083 HIV p24 hKIM-1 (pending) HLA-DR HLA-G HMB45 HNF1β (pending) Hotchkiss-McManus (pending) HPC2 HPL HPV HRAS HSD3B1/HSD3B2 hSNF5 Hsp90 (pending) HSV (pending) HTLV-1 (pending)

ICAM-1 ICE IDH1 IFITM1 (pending) IgG4 IgD (pending) IgG (pending) IgH IgM (pending) IL8 IMP3 (pending) ING1 ING2 ING3 Inhibin A Inhibin B INI1 INSM1 Iodine (pending) insulin (pending) integrins interleukins IRF4 ISL1 iron involucrin (pending)

JAK Jones methenamine silver (JMS) jun junB

Kappa Ki67 Kip1 KIT / CD117 KIT / CD117(melanoma) Kras KSHV Ku70 Ku80

Lambda laminin LANA LC3B lecithin lectins Leder LEF-1 (pending) Leu7 LFABP (pending) LIN28 Lipase (pending) lipid lipochrome LMO2 LMP Luxol fast blue lysozyme

MAC387 Machiavello (pending) MALT1 mammaglobin MAP2 (pending) MART1 Martius maspin MAST2 May-Grunwald-Giemsa MCL1 (pending) MDM2 mdr MED12 MEF2B MelanA melanin menin mesothelin metalloproteinases (MMP) methylene blue (pending) MGMT MHC class II (pending) MIB1 mic2 microphthalmia transcription factor (MITF) microsatellite instability MLH1 MLL MOC31 MRP MRP2 (pending) MSA MSH1 MSH2 MSH6 MTAP (pending) mTOR mucins MUC1 MUC2 MUC3 MUC4 (pending) MUC5AC MUC6 MUC7 (pending) multigene products (pending) MUM1 MYB MYC myeloperoxidase MyoD1 myogenin myoglobin myosin

NB84 (pending) NADH (pending) N-CAM NCCT (pending) n-myc NapsinA NEK2 nephrin NESP55+ (pending) Nestin (pending) NeuN neurofilament neuron specific enolase NF1 / neurofibromin NF1 (melanoma) NF2 NF-KB NKI-C3 NKX2 NKX3.1 nonspecific esterase NPM-ALK NRAS NTRK nucleophosmin NUTM1

Occludin p (pending) OCT2 (pending) OCT3/4 Oil Red O Olig2 OPA1 ORM-1 (pending) Osteocalcin (pending)

P glycoprotein p16 p21 p27 p40 p53 p57 p63 p120 catenin P504S Pan-TRK pancreatic polypeptide PAP PARP Parvalbumin (pending) PAS PASH (pending) PAX2 PAX3 (pending) PAX5 PAX7 PAX8 PBK PCA3 PCNA PD-1 PDGF PDL1 223C PDL1 SP142 (pending) PDS5B PDX1 (pending) PECAM1 Perforin (pending) Pentachrome PGP9.5 (pending) Pheochromocytoma (pending) PHF1 phosphohistone H3 Phospho-Smad2 (pending) PKD1 PKD2 PKD3 PLAG1 PLAP PLEKHA7 PMEL17 PML-RARα PMS2 podoplanin PPARγ Prealbumin (pending) PRKAR1A (pending) progesterone receptor/PR prostein PROX1 (pending) PSA PSAP PSMA PTAH PTEN PTEN (melanoma) PTH (pending) PU.1

RAD51 Rb RCC Reg IV renin RET reticulin retinoic acid ROS1 RRM1 RUNX1

S100 S100A1 (pending) S100P SALL4 SATB2 SDH (pending) SDHB selectin E selectin L selectin P serglycin (SRGN) serum amyloid A/SAA SF1 SGOL1 SHP-1 Sialyl-Tn silica Sirius red SIRT1 SMARCA4 SMMHC / smooth muscle myosin heavy chain smoothelin somatostatin (pending) SOX2 SOX9 SOX10 SOX11 SPan1 (pending) SSTR2A STAT3 STAT6 Steiner (pending) STMN1 (pending) Sudan Black B survivin SV40 (pending) synaptophysin SYT (pending)

TAK1/MAP3K7 tau T-bet TCL1 TCF4 (pending) TCRαβ (pending) TCRβF1 (pending) TCRγ (pending) TCRγδ (pending) TdT telomerase tenascin TFE3 TFF1 (pending) Thioflavin T (pending) Thioflavin S (pending) thrombomodulin thyroglobulin TIA1 TLE1 TLE3 (pending) Toluidine blue (pending) TOP2A TRAP TREM1 trichrome Trypsin (pending) tryptase TSC1/2 TTF1 tuberin tubulin TMB (pending) tumor necrosis factor twist1 tyrosinase

Ubiquitin unknown primary uric acid Uroplakin II (pending) uroplakin III

VCAM1 VEGF VEGF-D VEGFR3 VHL villin vimentin VIP (pending) vitronectin vWF VZW (Varicella-zoster virus) (pending)

Warthin-Starry wnt Wright-Giemsa WT1 WWTR1

YAP1

Zeb1 Ziehl-Neelsen ZO-1 (pending) Cell cycle

Superpages: entire chapter images virtual slides