Bone marrow nonneoplastic
General
Routine stains
Author: Nat Pernick, M.D.
Last author update: 1 December 2006
Last staff update: 14 May 2021
Copyright: 2002-2023, PathologyOutlines.com, Inc.
PubMed Search: Bone marrow routine stains
Table of Contents
Definition / generalCite this page: Pernick N Routine stains. PathologyOutlines.com website. https://www.pathologyoutlines.com/topic/bonemarrowroutinestains.html. Accessed June 3rd, 2023.
Definition / general
- Note: reactivity varies by type of fixation
- CD45 / LCA: lymphocytes
- Kappa and lambda light chains: to determine clonality or not of lymphocytes and plasma cells
- Myeloperoxidase and lysozyme: for AML subtyping
- B cell markers: CD20, CD79a and possibly PAX5
- T cell marker: CD3
- TdT: for ALL
- Iron stains (Prussian blue): on aspirate smears and trephine biopsies (J Clin Pathol 2005;58:269)
- 0 = Absent
- 1 = Trace
- 2 = Present (sparse)
- 3 = Present (moderate)
- 4 = Abundant (abnormal)
- To assess iron stores, examine 7 - 9 particles (J Clin Pathol 2004;57:1038)
- Absence of staining iron is not diagnostic of iron deficiency anemia (Ann Hematol 2001;80:166)
- Possibly reticulin stain or Masson trichrome stain
- Also CD34 (blasts and blood vessels), Factor VIII (blood vessels), CD61 (megakaryocytes), CD68 (macrophages), Hemoglobin A (for AML-M6) and glycophorin (for erythroid cells)
