Cervix

Cytology

Preparations



Last author update: 9 April 2019
Last staff update: 19 May 2022

Copyright: 2019-2022, PathologyOutlines.com, Inc.

PubMed Search: Cervix cytology preparations full text[sb]

Nat Pernick, M.D.
Marilin Rosa, M.D.
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Cite this page: Pernick N, Rosa M. Preparations. PathologyOutlines.com website. https://www.pathologyoutlines.com/topic/cervixcytologypreparations.html. Accessed July 5th, 2022.
Diff-Quik stain
Definition / general
Terminology
  • Also spelled Diff - Quick
Liquid based cytology
Technique
  • Head of spatula, where cells are lodged, is broken off into small glass vial containing preservative fluid or rinsed directly into preservative fluid
  • Sample is sent to lab, then spun and treated to remove mucus, pus or other obscuring material
  • Random sample of remaining cells is taken and deposited onto a slide

Clinical features
Clinical images

Images hosted on other servers:

Smearing and slide preparation

Papanicolaou (Pap) stain
Definition / general
  • Multichromatic staining histological technique developed by George Papanikolaou
  • Classic form involves five dyes in three solutions (IHC World: Papanicolaou Stain (Pap Stain) Protocol [Accessed 9 April 2019], Wikipedia: Papanicolaou Stain [Accessed 9 April 2019])
  • An alcohol dried preparation that produces better nuclear detail than air fixation
  • Stains ribosomes blue green, particularly in parabasal cells, mesothelial cells and metaplastic squamous cells
  • Stains metabolically inactive cells pink, such as superficial cells
  • Stains keratinized cells or thick specimens orange (benign or malignant)
  • Must fix smear quickly and stain carefully; air dried smears are inadequate

Clinical images

Images hosted on other servers:

Smearing and slide preparation

Procedure for staining



Cytology images

Images hosted on other servers:

Conventional
smears: examples
of good cellularity

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