Ultrasound guided fine needle aspiration (USFNA)

Topic Completed: 1 March 2013

Revised: 3 January 2019, last major update March 2013

Copyright: (c) 2002-2018,, Inc.

PubMed Search: Equipment [title] ultrasound guided fine needle aspiration

Joseph D. Jakowski, M.D.
Susan Meanor, R.T., R.D.M.S.
Page views in 2019: 48
Page views in 2020 to date: 17
Cite this page: Jakowski J. D., Meanor S. Equipment. website. Accessed May 24th, 2020.
Desirable equipment and supplies for USFNA of superficial sites
  • Portable (laptop type) or small bedside ultrasound machine:
    • B mode for small parts (e.g. thyroid), breast, musculoskeletal
    • Electronic adjustment of focal zone and depth
    • Caliper accuracy of better than 2% or 0.5 mm
    • Cineloop up to 1 minute at 25 frames per second
    • Removable storage media (USB, CD, etc.)
    • DICOM (Digital Imaging and COmmunication in Medicine) standard
    • PACS (Picture Archiving and Communication System)
    • Color Flow Doppler with / without Power Doppler
  • Ultrasound transducer:
    • Linear array with multifrequency capabilities
    • Frequency ranges: 6 - 7 MHz to 10 - 12 MHz or higher
    • Penetration: 6 cm or deeper
  • Ultrasound coupling gel
  • Ultrasound standoff pad (aids in imaging very superficial or skin based target lesions)
  • Transducer covers
  • IV extension tubing
  • Needles of 27, 25, 23, 22 gauge, from 1.0, 1.5 and 3 inches long (2.5 to 9 cm)
  • Disposable plastic syringes
  • Skin disinfectant: alcohol pads (chlorohexidine or providine iodine)
  • Transducer disinfectant (see manufacture recommendations as improper cleaning may cause damage and void warranty)
  • Pen and pencils
  • Plastic flexible ruler (for measuring lesions such as breast mass distance from the nipple)
  • Surgical ink pen (for skin marking or insertion site marking)
  • Sterile 4 x 4 sponges (e.g. to hold post biopsy pressure at needle puncture site)
  • Gloves and safety glasses
  • Small metal probe (to check transducer and planed needle insertion site over target lesion if needed)
  • Aspiration syringe holder
  • Local anesthesia (e.g. 1% lidocaine without epinephrine), if needed
  • Drapes, sheets, gowns, pillows (to cover patient or biopsy site, to help position and comfort patient)
  • Ice bag (to send home with patient if needed)
  • Band aids
  • Post aspiration biopsy handout informational sheet
  • Sharps container
Supplies for FNA smear making, rapid interpretation and triage of FNA sample
  • Microscope
  • Frosted end glass microscope slides
  • Romanowsky type stains (e.g. Diff-Quik)
  • RPMI tissue culture media (for collection for flow cytometry studies, etc.)
  • Alcohol spray fixative or 95% ethanol solution for fixing slides for Papanicolaou staining
  • Formalin (for cell block of FNA rinse)
  • Tube rack (to secure and hold FNA needle rinse tubes)
  • Various size collection tubes to place fluids / FNA material or for special studies (e.g. thyroglobulin washout study)
  • Sterile cup (to place aspirate material for culture)
  • Sterile saline syringe (to add a few drops to the FNA material in the sterile cup to prevent drying during transport)
  • Slide holder
Clinical images

Images hosted on PathOut server:
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Basic supplies

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Ultrasound supplies

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Post aspiration

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Rapid interpretation

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