Methods (molecular, IHC, frozen)
PCR thermocycling machines

Topic Completed: 1 July 2010

Minor changes: 15 February 2019

Copyright: 2008-2018,, Inc.

PubMed Search: PCR thermocycling machines

Rodney E. Shackelford, D.O., Ph.D.
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Table of Contents
Definition / general
Cite this page: Shackelford R. PCR thermocycling machines. website. Accessed January 18th, 2021.
Definition / general
  • Early PCR protocols required that each reaction be manually transferred to water baths of different temperatures at each temperature change
  • This problem was eliminated with the development of PCR thermocyclers, which rapidly change reaction temperatures without the need for manual transfers
  • The first machine for this purpose, "Mr. Cycle," was developed by Cetus Corporation
  • Since then, many different thermocyclers have been developed; most can be programmed to allow any desired temperature and time for each PCR cycle
  • More recent PCR machines can analyze multiple parallel real time PCR reactions and analyze the data via various computer algorithms
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