Stains & CD markers
OCT 3/4

Editor-in-Chief: Debra L. Zynger, M.D.
Robert Terlević, M.D.
Semir Vranić, M.D., Ph.D.

Last author update: 23 January 2020
Last staff update: 18 June 2021

Copyright: 2002-2023,, Inc.

PubMed Search: OCT 3/4 [Title]

Robert Terlević, M.D.
Semir Vranić, M.D., Ph.D.
Page views in 2022: 9,373
Page views in 2023 to date: 915
Cite this page: Terlević R, Vranić S. OCT 3/4. website. Accessed February 8th, 2023.
Definition / general
Essential features
  • Marker of germ cells, not expressed in somatic tissues
  • Strong diffuse nuclear expression seen in seminoma (dysgerminoma) and embryonal carcinoma components of germ cell neoplasia, including metastatic disease
  • Can be expressed focally in advanced epithelial and lymphatic malignancy
  • Nuclear staining, typically intense and diffuse
  • Cytoplasmic only staining in non germ cell derived tumors
Uses by pathologists
  • Marker for primary and metastatic germ cell derived tumors of the testis and ovary and extragonadal sites
  • Highly sensitive and specific for primary intracranial germinomas (Am J Surg Pathol 2005;29:368)
Prognostic factors
Microscopic (histologic) images

Contributed by Monika Ulamec, M.D., Ph.D.
Missing Image

Normal seminiferous ducts

Missing Image

In situ germ cell neoplasia

Missing Image


Missing Image

Embryonal carcinoma

Contributed by Debra Zynger, M.D.

Seminoma: H&E

OCT 3/4

OCT 3/4

 Contributed by Epitomics
Missing Image

OCT 3/4

Positive staining - normal
Positive staining - disease
Negative staining
Board review style question #1
    Which of the following testicular germ cell tumors consistently stain with OCT 3/4?

  1. Choriocarcinoma
  2. Embryonal carcinoma
  3. Immature teratoma
  4. Mature teratoma
  5. Yolk sac tumor
Board review style answer #1
B. Embryonal carcinoma

Comment Here

Reference: OCT 3/4
Back to top
Image 01 Image 02