Stains & CD markers
Topoisomerase II alpha (TOP2A)

Topic Completed: 1 June 2010

Minor changes: 12 August 2021

Copyright: 2002-2022,, Inc.

PubMed Search: Topoisomerase II alpha[TI] OR TOP2A[TI] free full text[sb]

Semir Vranić, M.D., Ph.D.
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Cite this page: Vranić, S. Topoisomerase II alpha (TOP2A). website. Accessed January 21st, 2022.
Definition / general
  • The best tool and cutoff values for TOP2A assessment are unknown
  • The most commonly used method is TOP2A gene analysis by FISH (Pathol Res Pract 2010;206:156)
  • TOP2A protein expression by immunohistochemistry is also commonly used
Uses by pathologists
Microscopic (histologic) images

Contributed by Semir Vranic, M.D., Ph.D.

Breast carcinoma

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Breast carcinomas

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Hodgkin lymphoma

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Lung cancer: small cell (+) versus adenocarcinoma (focal +)

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Prostate, hormone insensitive

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Prostate, various

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Positive staining - normal
  • Tissues with proliferating cells, especially spermatocytes, germinal centers and proliferative endometrium (Mod Pathol 1997;10:168)
Positive staining - not malignant
Positive staining - malignant
Negative staining
  • Terminally differentiated tissues (because TOP2A is not expressed in G0 phase): cerebral cortex, skeletal muscle and nerve
  • Rare positive cells in breast, salivary gland and kidney (Mod Pathol 1997;10:168)
Molecular / cytogenetics images

Contributed by Semir Vranic, M.D., Ph.D.

TOP2A CEP17 FISH no gene amplification

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