CD Markers
CD3

Author: Nat Pernick, M.D. (see Authors page)

Revised: 31 December 2015, last major update August 2013

Copyright: (c) 2002-2015, PathologyOutlines.com, Inc.

General
  • Common antibody for identifying T cells
  • Member of immunoglobulin superfamily on 11q23
  • Also called OKT3
  • Note: this topic also includes CD3 epsilon
Pathophysiology
  • Complex of delta (OMIM #186790), epsilon (OMIM #186830), gamma (OMIM #186740), zeta (OMIM #186780) and eta chains of integral membrane glycoproteins that associates with T cell antigen receptor (TCR), and is required for TCR cell surface expression and signal transduction (see Lymph nodes-not lymphoma chapter)
  • TCR-CD3 complex consists of either TCR alpha / beta or TCR gamma / delta heterodimers coexpressed at cell surface with CD3
Clinical Features
Uses by Pathologists
  • Most specific T cell antibody; usually to identify T cells in benign and malignant disorders; most antibodies are directed against epsilon chain
  • CD3 / CD20 immunostains are often performed in initial cytological evaluation of lymphoid-rich pleural effusions, but their cost-effectiveness has been questioned (Diagn Cytopathol 2012;40:565)
  • Does NOT improve detection of gluten sensitive enteropathy when H&E sections are normal.(Mod Pathol 2013;26:1241)
Micro Images
Images hosted on other servers:

Tonsil (normal)


Staining of tumor cells - images hosted on PathOut server:

Breast: high grade peripheral T cell lymphoma

Nose: NK/T cell lymphoma, nasal type

Thymoma: lymphocyte predominant


Staining of tumor cells - images hosted on other servers:

Liver:hepatosplenic
gamma-delta T cell
lymphoma (fig A)

Testis: NK/T cell lymphoma, nasal type

Pleural effusion:
primary effusion lymphoma
T cell type (fig B)


Staining of tumor cells - images hosted on Nature.com:

Lymph node: nodal CD8+ cytotoxic T cell lymphoma

Skin: mycosis fungoides


Staining of nonneoplastic infiltrating T cells - images hosted on PathOut server:

CNS: primary diffuse large B cell lymphoma

Eye: ocular adnexal mantle cell lymphoma

Lymph node: nodular lymphocyte predominant Hodgkin Lymphoma

Lymph node: nodal hamartoma

Myeloid sarcoma

Skin: halo nevus

Skin graft rejection


Staining of nonneoplastic infiltrating T cells - images hosted on other servers:

CNS: primary diffuse large B cell lymphoma

Esophagus diffuse large B cell lymphoma

Myeloid sarcoma

Skin: diffuse large B cell lymphoma (fig C)

Skin: Jessners lymphocytic infiltration


Inflammatory disorders - staining of T cells - images hosted on other servers:

Myocardium: acute cellular rejection

Thyroid: Hashimoto thyroiditis (fig c, e , f)


Inflammatory disorders - staining of T cells - images hosted on Nature.com:

Duodenum: intraepithelial lymphocytes left (fig b), right (fig c, d)


Negative controls / no T cells identified - image hosted on PathOut servers:

Stomach: MALT lymphoma


Negative controls / no T cells identified - image hosted on other servers:

Thyroid: MALT lymphoma is CD3- (fig d)

Positive Staining - Normal
  • Thymocytes, peripheral T cells (cytoplasmic expression at early T cell differentiation, then membranous expression)
  • NK cells (CD3 epsilon, cytoplasmic in 56%, not membranous); also Purkinje cells of cerebellum
  • Note: non-specific cytoplasmic staining may be present in plasma cells and macrophages
Positive Staining - Disease
  • Polykaryocytes in various benign and malignant disorders
  • 80% of T cell lymphomas, NK lymphoma (cytoplasmic, not membranous), lymphomatoid granulomatosis, lymphomatoid papulosis and pre T ALL (cytoplasmic staining)
  • LP cells in nodular lymphocyte predominant Hodgkin lymphoma
  • Variable in primary effusion lymphoma and pyothorax associated lymphoma
Negative Staining
  • B cells, histiocytes and histiocytic lesions, osteoblasts
  • Most B cell lymphomas, but aberrant staining exists (Am J Surg Pathol 2012;36:1364)
  • Aberrant loss of CD3 in some cases of mycosis fungoides (Am J Clin Pathol 2000;114:467), anaplastic large cell lymphoma and angioimmunoblastic T cell lymphoma (Am J Clin Pathol 2006;126:29)
  • NK large granular lymphocyte leukemia
  • Reed-Sternberg cells are negative in classic Hodgkin lymphoma but may be surrounded by CD3 epsilon+ rosettes; LP cells in nodular lymphocyte predominant Hodgkin lymphoma are CD3-
  • Small cell carcinoma, melanoma, granulocytic sarcoma, Ewing sarcoma and post-transplant lymphoproliferative disorders
Flow Cytometry Description
Flow Cytometry Images

Soft tissue: anaplastic large cell lymphoma