Skin nontumor

Topic Completed: 1 August 2010

Minor changes: 18 March 2020

Copyright: 2002-2020,, Inc.

PubMed Search: Grossing

Cecilia Rosales, M.D.
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Table of Contents
Definition / general
Cite this page: Rosales C. Grossing. website. Accessed May 27th, 2020.
Definition / general
  • Breadloafing (serial section) of ellipses is often best cut perpendicular to long axis at 3 mm intervals
  • Always ink margins for tumors, including deep margin
  • Bisect punch biopsies if 4 mm or larger

  • Biopsy for alopecia: can be cut perpendicular or horizontal depending of the pathology

  • Tzanck preparation (smear):
    • Used to identify Tzanck (multinucleated) cells of herpes simplex, Varicella and herpes zoster, pemphigus vulgaris or cytomegalovirus
    • Scrape the base of the blister / ulcer with the round base of the scalpel
    • Smear on a glass slide
    • Air-dry and stain with Wright's or Giemsa stains

  • Scabies (scrapping):
    • Place a drop of immersion oil on a microscope slide
    • Place a drop of immersion oil on the scalpel
    • Scrape the stratum corneum off the surface of the lesion
    • Numerous lesions should be scraped and placed on the same microscope slide

  • Submit fresh tissue for:
    • Immunofluorescence (can also use Michels medium)
    • Bacterial, fungal, and viral cultures
    • Chromosomal analysis
    • Antigen mapping of basement membrane zone / BMZ (saline splitting)
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