Skin-nontumor / Clinical Dermatology

Home Chapter Home Jobs Conferences Fellowships Books

Skin-nontumor / Clinical Dermatology

General

Grossing

Reviewer: Cecilia Rosales, M.D, Baylor College (see Reviewers page)
Revised: 24 August 2011, last major update August 2010
Copyright: (c) 2002-2011, PathologyOutlines.com, Inc.

General
=========================================================================

● Breadloafing (serial section) of ellipses is often best – cut perpendicular to long axis at 3 mm intervals
● Always ink margins for tumors, including deep margin
● Bisect punch biopsies if 4 mm or larger

● Biopsy for alopecia: can be cut perpendicular or horizontal depending of the pathology

● Tzanck preparation (smear):
    - Used to identify Tzanck (multinucleated) cells of herpes simplex, Varicella and herpes zoster, pemphigus vulgaris or cytomegalovirus
    - Scrape the base of the blister / ulcer with the round base of the scalpel
    - Smear on a glass slide
    - Air-dry and stain with Wright's or Giemsa stains (Family Practice Notebook)

● Scabies (scrapping):
   - Place a drop of immersion oil on a microscope slide
   - Place a drop of immersion oil on the scalpel
   - Scrape the stratum corneum off the surface of the lesion
   - Numerous lesions should be scraped and placed on the same microscope slide

● Submit fresh tissue for:
   - Immunofluorescence (can also use Michel’s medium)
   - Bacterial, fungal, and viral cultures
   - Chromosomal analysis
   - Antigen mapping of basement membrane zone / BMZ (saline splitting)

End of Skin-nontumor / Clinical Dermatology > General > Grossing

This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must be interpreted in the context of a patient's clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician.

All information on this website is protected by copyright of PathologyOutlines.com, Inc. Information from third parties may also be protected by copyright. Please contact us at copyrightPathOut@gmail.com with any questions (click here for other contact information).