Pathology Outlines

Stains
TTF1

Author: Nat Pernick, M.D. (see Authors page)

Revised: 14 February 2017, last major update June 2005

Copyright: (c) 2003-2017, PathologyOutlines.com, Inc.

PubMed Search: TTF1

Table of Contents

Cite this page: TTF1. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsttf1.html. Accessed July 23rd, 2018.

Definition / general

Thyroid transcription factor, also called thyroid specific enhancer binding protein
38 kDa nuclear protein that regulates transcription activity of thyroid (thyroglobulin, thyroperoxidase, sodium-iodide transport protein, calcitonin and MHC class I), lung (surfactant proteins A, B and C, Clara cell secretory protein) and diencephalon specific genes
Mutations cause pulmonary hypoplasia and neonatal death
Specificity depends on antibody clone used
Interpretation: nuclear stain

Uses by pathologists

Distinguish primary (TTF1+) vs. metastatic (usually TTF1-) lung carcinoma
Distinguish pulmonary adenocarcinoma (TTF1+) from squamous cell carcinoma (usually TTF1-)
Pleural lung carcinoma (TTF1+) vs. mesothelioma (TTF1-)
Pulmonary small cell carcinoma (TTF1+) vs. Merkel cell carcinoma (TTF1-)

Microscopic (histologic) images

Images hosted on PathOut server:

Contributed by Andrey Bychkov, M.D., Ph.D.:

Expression by follicular epithelium

Papillary thyroid carcinoma, TTF1

Images hosted on other servers:

Primary lung adenocarcinoma (TTF1+)

Lung metastasis to colon (TTF1+)

Pulmonary adenocarcinoma
metastasis (left) to
renal cell carcinoma (right)
CK7 (2A); TTF1+ (2B); RCC (2C)

Positive staining - normal

Lung type II pneumocytes and Clara cells; thyroid follicular and parafollicular C cells

Positive staining - disease

Lung carcinoma: small cell (90%), adenocarcinoma (75%); large cell (40%); squamous cell (5%, Am J Surg Pathol 2001;25:363)
Thyroid: hyperplastic and neoplastic thyroid tissue, but less common / lower levels in undifferentiated thyroid carcinomas (Mod Pathol 2000;13:570)
Other tumors: small cell carcinomas of lung and various sites (Mod Pathol 2000;13:238; Am J Surg Pathol 2001;25:815); colorectal carcinomas to lung may be positive (Mod Pathol 2005;18:1371), small cell carcinoma of bladder (39%, Hum Pathol 2005;36:718)

Negative staining

Merkel cell carcinoma of skin, most nonpulmonary non-small cell carcinomas